{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T14:46:23Z","timestamp":1758638783346,"version":"3.40.5"},"reference-count":62,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T00:00:00Z","timestamp":1696550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study evaluated the maternal, embryotoxic, and teratogenic effects of the aqueous extract of <jats:italic>Casearia sylvestris<\/jats:italic> (AECS), a species listed in the Unique Health System of Brazil, and widely used for treating several conditions, such as diarrhea, wounds, pain, and ulcers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Pregnant rats were daily treated orally with 0, 175, 350, or 700\u2009mg\/kg\/body weight of AECS, from gestational day (GD) 6 to 15 (organogenesis period). On GD 20, the pregnant rats were euthanized, and the litters submitted to an assessment of fetal development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No clinical signs of toxicity were observed in the dams during the treatment. In the embryo\u2010fetal development study, a significant increase in the basal zone height of the placenta was observed in the intermediate dose group. Furthermore, there was a significant increase in the relative anogenital distance measurement of female fetuses in the lowest and intermediate dose groups. Although no visceral abnormalities were observed in the treated\u2010fetuses, skeletal anomalies evidenced by changes in the ossification of the sternum and the presence of supernumerary ribs were found in the intermediate and high dose groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In conclusion, the treatment with AECS during organogenesis at this dose level had detrimental effects on the normal development of fetuses.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2257","type":"journal-article","created":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T17:47:03Z","timestamp":1696614423000},"page":"1899-1911","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Maternal\u2013fetal safety evaluation of an aqueous extract of <i>Casearia sylvestris<\/i> leaves in rats"],"prefix":"10.1002","volume":"115","author":[{"given":"Livia Trippe","family":"Nagaoka","sequence":"first","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biosciences of Botucatu, Universidade Estadual Paulista \u2013 UNESP  Botucatu S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara Campos","family":"Jorge","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biosciences of Botucatu, Universidade Estadual Paulista \u2013 UNESP  Botucatu S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00falia","family":"Stein","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biosciences of Botucatu, Universidade Estadual Paulista \u2013 UNESP  Botucatu S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz de Matos","family":"Manoel","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biosciences of Botucatu, Universidade Estadual Paulista \u2013 UNESP  Botucatu S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leticia Cardoso","family":"Valente","sequence":"additional","affiliation":[{"name":"College of Health Science, Federal University of Grande Dourados  Dourados Mato Grosso do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura Leonel","family":"de Toledo","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biosciences of Botucatu, Universidade Estadual Paulista \u2013 UNESP  Botucatu S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago Luis Aguayo","family":"de Castro","sequence":"additional","affiliation":[{"name":"Center of Studies in Natural Resources State University of Mato Grosso do Sul (UEMS)  Dourados Mato Grosso do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e2ndida Aparecida Leite","family":"Kassuya","sequence":"additional","affiliation":[{"name":"College of Health Science, Federal University of Grande Dourados  Dourados Mato Grosso do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Andrea Lima","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Center of Studies in Natural Resources State University of Mato Grosso do Sul (UEMS)  Dourados Mato Grosso do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2373-9399","authenticated-orcid":false,"given":"Arielle Cristina","family":"Arena","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology Institute of Biosciences of Botucatu, Universidade Estadual Paulista \u2013 UNESP  Botucatu S\u00e3o Paulo Brazil"},{"name":"Toxicological Information and Assistance Center (CIATOX), Institute of Biosciences of Botucatu, Univ. 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