{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T20:39:06Z","timestamp":1773693546970,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,30]],"date-time":"2023-12-30T00:00:00Z","timestamp":1703894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20192BAB205027"],"award-info":[{"award-number":["20192BAB205027"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>The purpose of this study is to explore the regulatory function of COL1A1 against the apoptosis of embryonic stem cells (ESCs) and the potential function in congenital talipes equinovarus (CTEV).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Muscle tissues were collected from 20 children with CTEV and 20 children without CTEV, followed by detecting the expression of COL1A1 using the RT\u2010PCR method. COL1A1 was knocked down in H1 and H9 human ESCs using the RNA interference technology, followed by determining the level of COL1A1, PITX1, TBX4, HOXD10, Fas, FasL, and Bax using the Western blotting assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>COL1A1 was found markedly upregulated in muscle tissues of CTEV children. In H1 and H9 human ESCs, compared to the empty vector, COL1A1, PITX1, TBX4, HOXD10, Fas, FasL, and Bax were found notably downregulated after transfected with the siRNA targeting COL1A1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>COL1A1 induced the apoptosis of ESCs by mediating the PITX1\/TBX4 signaling and might be a potential target for treating CTEV.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2277","type":"journal-article","created":{"date-parts":[[2023,12,30]],"date-time":"2023-12-30T08:19:20Z","timestamp":1703924360000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>COL1A1<\/scp> regulates the apoptosis of embryonic stem cells by mediating the <scp>PITX1<\/scp>\/<scp>TBX4<\/scp> signaling"],"prefix":"10.1002","volume":"116","author":[{"given":"Xiangping","family":"Du","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Jiangxi Provincial Children's Hospital  Nanchang City Jiangxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinle","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Jiangxi Provincial Children's Hospital  Nanchang City Jiangxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lirong","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Genetics and Metabolism Jiangxi Provincial Children's Hospital  Nanchang City Jiangxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wu","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Jiangxi Provincial Children's Hospital  Nanchang City Jiangxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Jiangxi Provincial Children's Hospital  Nanchang City Jiangxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fadi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Jiangxi Provincial Children's Hospital  Nanchang City Jiangxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Jiangxi Provincial Children's Hospital  Nanchang City Jiangxi Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2010.06.010"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejhg.2012.177"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmg.2014.06.001"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.95B12.32173"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmg.2017.09.006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.8422"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.18893"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-017-5290-0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.33130"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2008.10.004"},{"key":"e_1_2_8_12_1","first-page":"677","article-title":"Apoptotic genes expression in placenta of clubfoot\u2010like fetus pregnant rats","volume":"7","author":"Jiang B.","year":"2014","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.3119"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.17772\/gp\/60550"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.017384"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00147.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-018-0913-z"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.36238"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12833"},{"key":"e_1_2_8_20_1","first-page":"4861","article-title":"Relationship between HOX gene and pediatric congenital clubfoot","volume":"15","author":"Wang Y.","year":"2018","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.34167"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-016-4788-1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-016-2353-8"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.4238\/gmr15048773"}],"container-title":["Birth Defects Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdr2.2277","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T05:27:29Z","timestamp":1720675649000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdr2.2277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,30]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1002\/bdr2.2277"],"URL":"https:\/\/doi.org\/10.1002\/bdr2.2277","archive":["Portico"],"relation":{},"ISSN":["2472-1727","2472-1727"],"issn-type":[{"value":"2472-1727","type":"print"},{"value":"2472-1727","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,30]]},"assertion":[{"value":"2023-08-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2277"}}