{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T11:35:04Z","timestamp":1763811304314,"version":"3.41.2"},"reference-count":62,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,25]],"date-time":"2023-12-25T00:00:00Z","timestamp":1703462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Tetralogy of Fallot (TOF) is the most common cyanotic congenital heart defect in the United States. We aimed to identify genetic variations associated with TOF using meta\u2010analysis of publicly available digital samples to spotlight targets for prevention, screening, and treatment strategies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used the Search Tag Analyze Resource for Gene Expression Omnibus (STARGEO) platform to identify 39 TOF and 19 non\u2010TOF right ventricle tissue samples from microarray data and identified upregulated and downregulated genes. Associated gene expression data were analyzed using ingenuity pathway analysis and restricted to genes with a statistically significant (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) difference and an absolute experimental log ratio &gt;0.1 between disease and control samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our analysis identified 220 genes whose expression profiles were significantly altered in TOF vs. non\u2010TOF samples. The most striking differences identified in gene expression included genes FBXO32, PTGES, MYL12a, and NR2F2. Some top associated canonical pathways included adrenergic signaling, estrogen receptor signaling, and the role of NFAT in cardiac hypertrophy. In general, genes involved in adaptive, defensive, and reparative cardiovascular responses showed altered expression in TOF vs. non\u2010TOF samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We introduced the interpretation of open \u201cbig data\u201d using the STARGEO platform to define robust genomic signatures of congenital heart disease pathology of TOF. Overall, our meta\u2010analysis results indicated increased metabolism, inflammation, and altered gene expression in TOF patients. Estrogen receptor signaling and the role of NFAT in cardiac hypertrophy represent unique pathways upregulated in TOF patients and are potential targets for future pharmacologic treatments.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2293","type":"journal-article","created":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T05:24:13Z","timestamp":1703568253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>Meta\u2010analysis<\/scp> reveals differential gene expression in tetralogy of Fallot versus controls"],"prefix":"10.1002","volume":"116","author":[{"given":"Sarah Mae","family":"Voskamp","sequence":"first","affiliation":[{"name":"University of Central Florida College of Medicine  Orlando Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maya Alexis","family":"Hammonds","sequence":"additional","affiliation":[{"name":"University of Central Florida College of Medicine  Orlando Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas M.","family":"Knapp","sequence":"additional","affiliation":[{"name":"University of Central Florida College of Medicine  Orlando Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley L.","family":"Pekmezian","sequence":"additional","affiliation":[{"name":"University of Central Florida College of Medicine  Orlando Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dexter","family":"Hadley","sequence":"additional","affiliation":[{"name":"University of Central Florida College of Medicine  Orlando Florida USA"},{"name":"Department of Clinical Sciences University of Central Florida College of Medicine  Orlando Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9656-1125","authenticated-orcid":false,"given":"Jennifer S.","family":"Nelson","sequence":"additional","affiliation":[{"name":"University of 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