{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:04:08Z","timestamp":1753862648554,"version":"3.41.2"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aims to determine if 5,10\u2010methylenetetrahydrofolate reductase (MTHFR C677T and A1298C) and methionine synthase reductase (MTRR A66G) gene polymorphisms were associated with fatty acid (FA) levels in mothers of fetuses with neural tube defects (NTDs) and whether these associations were modified by environmental factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma FA composition was assessed using capillary gas chromatography. Concentrations of studied FA were compared between 42 mothers of NTDs fetuses and 30 controls as a function of each polymorphism by the Kruskal\u2013Wallis nonparametric test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In MTHFR gene C677T polymorphism, cases with (CT\u2009+\u2009TT) genotype had lower monounsaturated FA (MUFA) and omega\u20103 polyunsaturated FA (n\u20103 PUFA) levels, but higher omega\u20106 polyunsaturated FA (n\u20106 PUFA) and omega\u20106 polyunsaturated FA: omega\u20103 polyunsaturated FA (n\u20106:n\u20103) ratio levels. In MTRR gene A66G polymorphism, cases with (AG\u2009+\u2009GG) genotype had lower MUFA levels, but higher PUFA and n\u20106 PUFA levels. Controls with (AG\u2009+\u2009GG) genotype had lower n\u20106 PUFA levels. In MTHFR gene C677T polymorphism, cases with smoking spouses and (CT\u2009+\u2009TT) genotype had lower MUFA and n\u20103 PUFA levels, but higher PUFA, n\u20106 PUFA, and n\u20106:n\u20103 ratio levels. Cases with (CT\u2009+\u2009TT) genotype and who used sauna during pregnancy had lower n\u20103 PUFA levels. In MTRR gene A66G polymorphism, cases with (AG\u2009+\u2009GG) genotype and who used sauna during pregnancy had higher PUFA and n\u20106 PUFA levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Further research is required to clarify the association of FA metabolism and (MTHFR, MTRR) polymorphisms with NTDs.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2333","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T08:23:22Z","timestamp":1715156602000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Association of <scp>MTHFR<\/scp> (<scp>C677T<\/scp>, <scp>A1298C<\/scp>) and <scp>MTRR A66G<\/scp> polymorphisms with fatty acids profile and risk of neural tube defects"],"prefix":"10.1002","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9380-4013","authenticated-orcid":false,"given":"Kaouther","family":"Nasri","sequence":"first","affiliation":[{"name":"Faculty of Sciences of Bizerte University of Carthage  Bizerte Tunisia"},{"name":"Service of Embryo\u2010Fetopathology, Center for Maternity and Neonatology of Tunis Tunis El Manar University  Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia","family":"Ben Jamaa","sequence":"additional","affiliation":[{"name":"Department of Histology\u2010Embryology, Faculty of Medicine of Tunis Tunis El Manar University  Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soumeya Siala","family":"Gaigi","sequence":"additional","affiliation":[{"name":"Service of Embryo\u2010Fetopathology, Center for Maternity and Neonatology of Tunis Tunis El Manar University  Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moncef","family":"Feki","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Rabta Hospital, Tunis El Manar University Jebbari  Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raja","family":"Marrakchi","sequence":"additional","affiliation":[{"name":"Laboratory of Human Genetics, Immunology and Pathology, Faculty of Sciences of Tunis Tunis El Manar University  Tunis Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3223(02)01443-9"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2010.04.011"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199911113412001"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9121364"},{"key":"e_1_2_11_6_1","first-page":"1","article-title":"Recommendations for the use of folic acid to reduce the number of cases of spina bifida and other neural tube defects","volume":"41","author":"Centers for Disease Control","year":"1992","journal-title":"MMWR \u2010 Recommendations and Reports"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00311.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2011.09.020"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu6051993"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.g4554"},{"key":"e_1_2_11_11_1","first-page":"57","article-title":"Effect of cigarette smoking on blood lipids\u2013A study in Belgaum, northern Karnataka, India","volume":"6","author":"Devaranavadgi B. 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