{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:01:33Z","timestamp":1772118093621,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T00:00:00Z","timestamp":1715990400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cyprodinil is a widely used fungicide with broad\u2010spectrum activity, but it has been associated with cardiac abnormalities. (\u2212)\u2010Epicatechin gallate (ECG), a natural polyphenolic compound, has been shown to possess protective properties in cardiac development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, we investigated whether ECG could mitigate cyprodinil\u2010induced heart defects using zebrafish embryos as a model. Zebrafish embryos were exposed to cyprodinil with or without ECG.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our results demonstrated that ECG significantly improved the survival rate, embryo movement, and hatching delay induced by cyprodinil. Furthermore, ECG effectively ameliorated cyprodinil\u2010induced cardiac developmental toxicity, including pericardial anomaly and impairment of cardiac function. Mechanistically, ECG attenuated the cyprodinil\u2010induced alterations in mRNA expression related to cardiac development, such as\n                      <jats:italic>amhc<\/jats:italic>\n                      ,\n                      <jats:italic>vmhc<\/jats:italic>\n                      ,\n                      <jats:italic>tbx5<\/jats:italic>\n                      , and\n                      <jats:italic>gata4<\/jats:italic>\n                      , as well as calcium ion channels, such as\n                      <jats:italic>ncx1h<\/jats:italic>\n                      ,\n                      <jats:italic>atp2a2a<\/jats:italic>\n                      , and\n                      <jats:italic>cdh2<\/jats:italic>\n                      . Additionally, ECG was found to inhibit the activity of the aryl hydrocarbon receptor (AhR) signaling pathways induced by cyprodinil.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In conclusion, our findings provide evidence for the protective effects of ECG against cyprodinil\u2010induced cardiac developmental toxicity, mediated through the inhibition of AhR activity. These findings contribute to a better understanding of the regulatory mechanisms and safe utilization of pesticide, such as cyprodinil.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/bdr2.2350","type":"journal-article","created":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T04:24:49Z","timestamp":1716006289000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["(\u2212)\u2010Epicatechin gallate ameliorates cyprodinil\u2010induced cardiac developmental defects through inhibiting aryl hydrocarbon receptor in zebrafish"],"prefix":"10.1002","volume":"116","author":[{"given":"Dongqin","family":"Huang","sequence":"first","affiliation":[{"name":"Neonatology Anxi County Hospital  Quanzhou People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchao","family":"Su","sequence":"additional","affiliation":[{"name":"Neonatology Anxi County Hospital  Quanzhou People's Republic 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