{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:04:02Z","timestamp":1753862642274,"version":"3.41.2"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T00:00:00Z","timestamp":1718236800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2020J011128","2023J011341"],"award-info":[{"award-number":["2020J011128","2023J011341"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In addition to the potential for multiple pregnancies, natural conception occurring in preimplantation genetic testing (PGT) increases undesired genetic risk. Some studies showed that a dichorionic diamniotic twin pregnancy after a single blastocyst transfer could be caused by embryo splitting or concurrent spontaneous conception.<\/jats:p><\/jats:sec><jats:sec><jats:title>Case<\/jats:title><jats:p>We describe a patient undergoing PGT who had a dichorionic diamniotic twin pregnancy after single blastocyst transfer in a natural cycle. In this case, we recommended to determine genetic status of the twins by prenatal diagnosis. The results showed that karyotype, chromosome copy number variation, and parental <jats:italic>ACAT1<\/jats:italic> variation of the twins were all normal and similar. To investigate the origin of pregnancy, we used the genotype data of single\u2010nucleotide polymorphisms typical of genome\u2010wide association studies. Dizygotic twins were inferred by robust estimation of kinship coefficients, which confirmed the occurrence of a spontaneous conception.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This case strengthens the importance of genetic counseling to inform couples with reproductive genetic risk, such as those who undergo PGT, that intercourse should be avoided, especially in natural transfer cycles. Moreover, prenatal diagnosis remains essential and is strongly recommended to avoid genetic risks.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2366","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T06:58:51Z","timestamp":1718348331000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dichorionic diamniotic twin pregnancy after preimplantation genetic testing and single blastocyst transfer"],"prefix":"10.1002","volume":"116","author":[{"given":"Qiuxiang","family":"Huang","sequence":"first","affiliation":[{"name":"Center of Reproductive Medicine, Department of Obstetrics and Gynecology Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Support Force)  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Yan","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Department of Obstetrics and Gynecology Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Support Force)  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Mao","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Department of Obstetrics and Gynecology Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Support Force)  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caixia","family":"Wang","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Department of Obstetrics and Gynecology Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Support Force)  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Lin","sequence":"additional","affiliation":[{"name":"Laboratory of Basic Medicine Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Support Force)  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5021-4478","authenticated-orcid":false,"given":"Yun","family":"Liu","sequence":"additional","affiliation":[{"name":"Center of Reproductive Medicine, Department of Obstetrics and Gynecology Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Support Force)  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2166-6576","authenticated-orcid":false,"given":"Zhihong","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Basic Medicine Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Support Force)  Fuzhou 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