{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:34:22Z","timestamp":1788435262222,"version":"build-2803163510"},"reference-count":99,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T00:00:00Z","timestamp":1730332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Miscarriages cause a greater loss\u2010of\u2010life than cardiovascular diseases, but knowledge about environmentally induced miscarriages is limited. Cultured na\u00efve pluripotent embryonic stem cells (ESC) differentiate into extra\u2010embryonic endoderm\/extraembryonic endoderm (XEN) or formative pluripotent ESC, during the period emulating maximal miscarriage of peri\u2010implantation development. In previous reports using small marker sets, hyperosmotic sorbitol, or retinoic acid (RA) decreased na\u00efve pluripotency and increased XEN by FACS quantitation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bulk and single cell (sc)RNAseq analyses of two cultured ESC lines was done, corroborated by qPCR. Transcriptomic responses were analyzed of cultured ESC stressed by Sorbitol, with Leukemia inhibitory factor (LIF\u2009+\u2009; stemness growth factor), RA without LIF to control for XEN induction, and compared with normal differentiation (LIF\u2009\u2212\u2009, ND).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sorbitol and RA increase subpopulations of 2\u2010cell embryo\u2010like (2CEL) and XEN sub\u2010lineages; primitive, parietal, and visceral endoderm (VE) cells and suppress formative pluripotency, imbalancing alternate lineage choices of initial na\u00efve pluripotent cultured ESC compared with ND. Although bulk RNAseq and gene ontology (GO) group analyses suggest that stress induces anterior VE\u2010head organizer and placental markers, scRNAseq reveals relatively few cells. But VE and placental markers\/cells were in adjacent stressed cell clusters in the UMAP, like recent, normal UMAP of conceptuses. UMAPs show that dose\u2010dependent stress overrides stemness to force premature lineage imbalance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Hyperosmotic stress, and other toxicological stresses, like drugs with active ingredient RA, may cause premature, lineage imbalance, resulting in miscarriages or birth defects.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2409","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T04:15:09Z","timestamp":1730434509000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Single Cell Transcriptomic Fingerprint of Stressed Premature, Imbalanced Differentiation of Embryonic Stem Cells"],"prefix":"10.1002","volume":"116","author":[{"given":"Ximena","family":"L\u00a0Ruden","sequence":"first","affiliation":[{"name":"CS Mott Center\/Ob\/Gyn Department Wayne State University (WSU)  Detroit Michigan 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