{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T06:18:44Z","timestamp":1787897924751,"version":"build-2784847793"},"reference-count":74,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017678","name":"Loyola Marymount University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017678","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cardiac neural crest cells (cNCCs) are critical for heart development, and their disruption can result in congenital heart defects. Serotonin (5\u2010HT) signaling, specifically via 5\u2010HT2B and 5\u2010HT2C receptors, regulates diverse physiological processes, including neural crest migration. This study investigates how modulation of 5\u2010HT2B and 5\u2010HT2C receptor activity impacts cNCC migration and the development of their derivatives, with relevance to serotonergic drug safety during pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Chicken embryos at HH8 were treated with 50\u2009\u03bcL of 20\u2009\u03bcM 1\u2010Methylpsilocin (1\u2010MP), an inverse agonist of 5\u2010HT2B and agonist of 5\u2010HT2C, and collected at HH14 to assess cNCC migration. Embryos were pre\u2010treated with SB242084, a selective 5\u2010HT2C antagonist, to isolate receptor\u2010specific contributions before 1\u2010MP application. Phenotypic outcomes were assessed at HH32 and HH36 for structural heart defects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>1\u2010MP disrupted cNCC migration at HH14, evidenced by abnormal shortening of the circumpharyngeal neural crest (CirNCC) stream. Pre\u2010treatment with SB242084 did not rescue the phenotype, implicating 5\u2010HT2B as the primary driver, though potential contributions from 5\u2010HT2C cannot be excluded. At HH32, 1\u2010MP\u2010treated embryos displayed gaps in the aorticopulmonary septum. By HH36, interventricular septal defects and delayed development further supported the role of 5\u2010HT2B in cNCC migration and differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings reveal that 5\u2010HT2B receptor activity is critical for cNCC migration and heart development. They underscore the potential teratogenic risks of serotonergic drugs targeting 5\u2010HT2B\/5\u2010HT2C receptors during pregnancy, with implications for drug safety and heart morphogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2506","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T02:15:04Z","timestamp":1752718504000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Teratogenic Effects of Serotonin Receptor <scp>2B<\/scp> Disruption on the Migration and Cardiac Derivatives of the Cardiac Neural Crest"],"prefix":"10.1002","volume":"117","author":[{"given":"Brian K.","family":"Wells","sequence":"first","affiliation":[{"name":"Biology Department Loyola Marymount University  Los Angeles California USA"},{"name":"School of Biological Sciences University of California San Diego  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwyneth K.","family":"Garramone","sequence":"additional","affiliation":[{"name":"Biology Department Loyola Marymount University  Los Angeles California USA"},{"name":"Program in Cell, Molecular, Development Biology, and Biophysics Johns Hopkins University  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Mahomed","sequence":"additional","affiliation":[{"name":"Biology Department Loyola Marymount University  Los Angeles California USA"},{"name":"School of Dentistry University of California Los Angeles  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Ezin","sequence":"additional","affiliation":[{"name":"Biology Department Loyola Marymount University  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Theory and Practice of Histological Techniques","author":"Bancroft J. D.","year":"2008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21051649"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092250306"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001800403"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.10099"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2013.01.007"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01197"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrb.20258"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.12.010"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.9.1745"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031\u20109384(03)00047\u20107"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9209com"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1997.0499"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.66.6.1491"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450\u2010199712000\u201000002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12471\u2010011\u20100226\u2010x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2017.01.011"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1071"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0893\u2010133X(02)00353\u20106"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.04.005"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/da.20735"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.188706"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2005.03.003"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.f6932"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050880104"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2011.03.008"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1312828"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI44244"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.1.387"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu8010056"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.8.1607"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450\u2010198703000\u201000001"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/00045415\u2010199507000\u201000006"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6844926"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092130112"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001910302"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.102.4.709"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-3860-4_28"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0736\u20105748(00)00032\u20100"},{"issue":"3","key":"e_1_2_7_41_1","first-page":"265","article-title":"Sites of Serotonin Uptake in Epithelia of the Developing Mouse Palate, Oral Cavity, and Face: Possible Role in Morphogenesis","volume":"8","author":"Lauder J. M.","year":"1988","journal-title":"Journal of Craniofacial Genetics and Developmental Biology"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.12.002"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.31989\/ffhd.v4i6.9"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.81.3.1047"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10264"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000215809.47923.fd"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00188.2016"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.22405"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2015.01.009"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21743"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.10.028"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.08.007"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.01.013"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.16.7182"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0736-5748(01)00022-3"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000081520.25714.D9"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10741\u2010016\u20109536\u20109"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23276"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1097\/JCP.0b013e3181dd68d2"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2005.06.104"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(94)00005-N"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.105445"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1177\/37.3.2918221"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13023\u2010014\u20100144\u20102"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520299109109990"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.12.041"},{"key":"e_1_2_7_68_1","volume-title":"Reference Module in Chemistry, Molecular Sciences and Chemical Engineering","author":"Townsend C.","year":"2020"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1159\/000088130"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.8860"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.02.011"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097\u20100177(199603)205:3<281::AID\u2010AJA8>3.0.CO;2\u2010E"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.61.3.571"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420470609"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcdd8100121"}],"updated-by":[{"DOI":"10.1002\/bdr2.2519","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000}}],"container-title":["Birth Defects Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdr2.2506","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T09:56:19Z","timestamp":1757325379000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdr2.2506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":74,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/bdr2.2506"],"URL":"https:\/\/doi.org\/10.1002\/bdr2.2506","archive":["Portico"],"relation":{"correction":[{"id-type":"doi","id":"10.1002\/bdr2.2519","asserted-by":"object"}]},"ISSN":["2472-1727","2472-1727"],"issn-type":[{"value":"2472-1727","type":"print"},{"value":"2472-1727","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2023-12-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2506"}}