{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T05:40:02Z","timestamp":1758865202850,"version":"3.44.0"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Corn (<jats:styled-content style=\"fixed-case\"><jats:italic>Zea mays<\/jats:italic><\/jats:styled-content> L., Poaceae) stigmas, popularly known as corn silk, have been traditionally used for their diuretic properties and to treat urinary tract disorders and hypertension. Although several studies have reported beneficial effects, such as anti\u2010inflammatory and antioxidant activities, data on their safety during pregnancy remain limited. This study aimed to evaluate the effects of prenatal treatment with a standardized dry corn silk extract on maternal and fetal toxicity parameters in Wistar rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Corn silk was extracted with 41% (v\/v) ethanol, concentrated, and spray\u2010dried. The total flavonoid content was determined by spectrophotometry, and phenolic compounds, including maysin, were analyzed using HPLC\u2010DAD and HPLC\u2010ESI\u2010MS\/MS. Pregnant Wistar rats (<jats:italic>n<\/jats:italic>\u2009=\u200910\/group) received the vehicle or extract doses of 300, 600, or 1200\u2009mg\/kg from gestational Day (GD) 0\u201320. Clinical signs of toxicity, body weight gain, and food and water intake were monitored. On GD 21, blood samples were collected for biochemical analysis. Reproductive performance and genotoxicity were assessed, along with histopathological examinations of selected organs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The extract contained 1.698% flavonoids, including maysin as the predominant one. No clinical signs of toxicity, changes in body weight gain, water and food intake, genotoxicity, histopathological alterations, or biochemical disturbances were observed. However, the 1200\u2009mg\/kg dose increased pre\u2010implantation losses. No external, skeletal, or visceral malformations were detected in fetuses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Under these experimental conditions, the extract did not induce maternal or fetal toxicity. Nevertheless, the 1200\u2009mg\/kg dose may interfere with early pregnancy in Wistar rats.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2526","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T07:33:20Z","timestamp":1758612800000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Toxicological Assessment of a Standardized Dry Extract of <scp><i>Zea mays<\/i><\/scp> L. (Poaceae) Stigmas During Gestation: Effects on Maternal Parameters and Fetal Outcomes in Wistar Rats"],"prefix":"10.1002","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5071-5077","authenticated-orcid":false,"given":"Graziele Al\u00edcia Batista","family":"Caixeta","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o Em Ci\u00eancias Aplicadas a Produtos Para Sa\u00fade (CAPS). 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