{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:19:44Z","timestamp":1790155184333,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000038","name":"U.S. Food and Drug Administration","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Perfluorooctane sulfonic acid (PFOS) belongs to a group of synthetic chemicals referred to as per\u2010 and polyfluoroalkyl substances (PFAS) that are known to degrade slowly. Exposure to PFOS has been reported to cause birth defects and disrupt female sex hormone levels in mice. However, to date, the direct effects of PFOS exposure on folliculogenesis in young animals have not been evaluated. Therefore, this study was conducted to examine the potential effects of in utero and prepubertal exposure to PFOS on follicle development in juvenile, female mouse pups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      ICR dams and young, female mouse pups were dosed with 0.1\u2009mg\/kg PFOS or saline (vehicle) via gavage beginning on gestation day (GD) 6 (plug positive\u2009=\u2009GD 0) through GD 17 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200911\u201317 per group) [in utero exposure] or postnatal day (PND) 7 (PND 0\u2009=\u2009day of birth) through PND 21 (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200922\u201329 per group) [prepubertal exposure]. Ovaries and blood were then collected from a pup\/litter on PND 28 for analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      There were no adverse effects of PFOS exposure observed in the measured endpoints upon in utero exposure; however, statistically significant differences following prepubertal exposure were noted for serum estradiol levels, the total number of primordial follicles, and the transcript levels of\n                      <jats:italic>Foxo1<\/jats:italic>\n                      and\n                      <jats:italic>Gdf9<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The preliminary findings suggest that prepubertal exposure to PFOS may affect follicle development by disrupting estradiol production and altering\n                      <jats:italic>Foxo1<\/jats:italic>\n                      and\n                      <jats:italic>Gdf9<\/jats:italic>\n                      gene expression. Further studies will be important to examine the mechanisms underlying prepubertal exposure to PFOS on folliculogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/bdr2.70052","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T10:04:36Z","timestamp":1778061876000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Potential Effects of Prepubertal Exposure to Perfluorooctane Sulfonic Acid on the First Wave of Folliculogenesis in Young\n                    <scp>CD<\/scp>\n                    \u20101 Mice"],"prefix":"10.1002","volume":"118","author":[{"given":"Bounleut","family":"Phanavanh","sequence":"first","affiliation":[{"name":"National Center for Toxicological Research, U.S. Food and Drug Administration  Jefferson Arkansas 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