{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:48:04Z","timestamp":1791085684120,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,12,5]],"date-time":"2006-12-05T00:00:00Z","timestamp":1165276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND:<\/jats:bold> CHARGE syndrome represents a constellation of malformations: C, coloboma of the iris or retina; H, heart defects; A, atresia of the choanae; R, retardation of growth and\/or development; G, genital anomalies; and E, ear abnormalities. Recently, the <jats:italic>Chromodomain helicase DNA\u2010binding protein\u20107<\/jats:italic> (<jats:italic>CHD7<\/jats:italic>) at chromosome 8q12.1 was identified as a causative gene for CHARGE syndrome. Because <jats:italic>CHD7<\/jats:italic> was identified as a causative gene using a positional cloning approach, the role of <jats:italic>CHD7<\/jats:italic> in early embryogenesis needs to be further investigated. <jats:bold>METHODS:<\/jats:bold> Fertilized chick eggs were incubated to Hamburger and Hamilton stages 4\u201320 and were studied using whole mount <jats:italic>in situ<\/jats:italic> hybridization. Chicken EST clones corresponding to the chicken <jats:italic>CHD7<\/jats:italic> (<jats:italic>cChd7<\/jats:italic>) sequence were identified using the chicken EST sequence database. From the EST clones, a digoxigenin\u2010labeled RNA probe was synthesized and hybridized <jats:italic>in situ<\/jats:italic> to the embryonic specimens. <jats:bold>RESULTS:<\/jats:bold> The expression of <jats:italic>cChd7<\/jats:italic> was pan\u2010neuronal at stages 8\u201320. It was expressed throughout the rostral neural ectoderm and along the rostrocaudal axis but was absent from the more lateral, non\u2010neuronal ectoderm. Adjacent to the neural tube, <jats:italic>cChd7<\/jats:italic> transcripts were detected at the optic and otic placodes. At stage 20, <jats:italic>cChd7<\/jats:italic> expression was observed in the branchial arches and olfactory placodes in addition to brain and optic and otic placodes. <jats:bold>CONCLUSIONS:<\/jats:bold><jats:italic>cChd7<\/jats:italic> was expressed in the neural epithelium, the otic placodes, the optic placodes, the branchial arches, and the olfactory placodes, which are the primordial tissues that give rise to organs affected in human CHARGE syndrome patients. Birth Defects Research (Part A), 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/bdra.20330","type":"journal-article","created":{"date-parts":[[2006,12,5]],"date-time":"2006-12-05T19:19:22Z","timestamp":1165346362000},"page":"50-57","source":"Crossref","is-referenced-by-count":38,"title":["Embryonic expression profile of chicken <i>CHD7<\/i>, the ortholog of the causative gene for CHARGE syndrome"],"prefix":"10.1002","volume":"79","author":[{"given":"Michihiko","family":"Aramaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tokuhiro","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Udaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rika","family":"Kosaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Mitsuhashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasunori","family":"Okada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenjiro","family":"Kosaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,12,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2005.10.044"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/000992289803700302"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)01296-4"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2004.04.020"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0290023"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2004-1970"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0146-0005(96)80062-0"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-8628(2000)9999:999<00::AID-AJMG1132>3.0.CO;2-J"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(79)80513-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050880104"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3928\/0191-3913-19790301-10"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03154"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2003.017640"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20468"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.18.187"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(81)80454-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(00)02024-2"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(89)90076-4"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1407"}],"container-title":["Birth Defects Research Part A: Clinical and Molecular Teratology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbdra.20330","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdra.20330","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T12:55:10Z","timestamp":1700052910000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdra.20330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12,5]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/bdra.20330"],"URL":"https:\/\/doi.org\/10.1002\/bdra.20330","archive":["Portico"],"relation":{},"ISSN":["1542-0752","1542-0760"],"issn-type":[{"value":"1542-0752","type":"print"},{"value":"1542-0760","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12,5]]}}}