{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T15:07:11Z","timestamp":1785683231403,"version":"3.56.0"},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,1,9]],"date-time":"2009-01-09T00:00:00Z","timestamp":1231459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>OBJECTIVES:<\/jats:bold> Following up on prior evidence from animal and human studies of Ljungan virus (LV) in intrauterine fetal death (IUFD), we examine additional cases of IUFD using two standard assays of viral detection: immunohistochemistry (IHC) and real time RT\u2010PCR. <jats:bold>MATERIALS AND METHODS:<\/jats:bold> Frozen and formalin\u2010fixed specimens from IUFD cases were tested for the presence of LV using real time RT\u2010PCR and IHC, respectively. Formalin\u2010fixed organs from terminated pregnancies diagnosed as trisomy 21 were used as controls in the IHC assay. <jats:bold>RESULTS:<\/jats:bold> Presence of LV was demonstrated in all five IUFD cases by IHC and further confirmed in three of these cases by real time RT\u2010PCR. Only one of 18 trisomy 21 controls was LV positive by IHC. <jats:bold>CONCLUSION:<\/jats:bold> The presence of LV in IUFD patients has been confirmed by two different assays. Birth Defects Research (Part A), 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/bdra.20554","type":"journal-article","created":{"date-parts":[[2009,1,9]],"date-time":"2009-01-09T20:59:52Z","timestamp":1231534792000},"page":"227-229","source":"Crossref","is-referenced-by-count":19,"title":["Ljungan virus present in intrauterine fetal death diagnosed by both immunohistochemistry and PCR"],"prefix":"10.1002","volume":"85","author":[{"given":"Annika","family":"Samsioe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikos","family":"Papadogiannakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Hultman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5ke","family":"Sj\u00f6holm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Klitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Niklasson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.11.029"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.48.1.43"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-12.2.154"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9557"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-006-0493-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20359"},{"key":"e_1_2_6_8_1","article-title":"Zoonotic ljungan virus associated with central nervous system malformations in terminated pregnancy","author":"Niklasson B","journal-title":"Birth Defects Res A Clin Mol Teratol"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdrb.20083"},{"key":"e_1_2_6_10_1","article-title":"Fetal death persists through recurrent pregnancies in mice following Ljungan virus infection","author":"Samsioe A","journal-title":"Birth Defects Research B Dev Reprod Toxicol"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704500301"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/39.6.1709656"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.02.012"}],"container-title":["Birth Defects Research Part A: Clinical and Molecular Teratology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbdra.20554","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdra.20554","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T23:11:40Z","timestamp":1699917100000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdra.20554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,9]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["10.1002\/bdra.20554"],"URL":"https:\/\/doi.org\/10.1002\/bdra.20554","archive":["Portico"],"relation":{},"ISSN":["1542-0752","1542-0760"],"issn-type":[{"value":"1542-0752","type":"print"},{"value":"1542-0760","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1,9]]}}}