{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:11:46Z","timestamp":1790107906148,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,6,8]],"date-time":"2011-06-08T00:00:00Z","timestamp":1307491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Hirschsprung disease (HSCR) is a congenital disorder characterized by an absence of intrinsic ganglion cells in the nerve plexuses of the lower colon. The Semaphorin 3A (<jats:italic>SEMA3A<\/jats:italic>) gene is involved in the migration of enteric neural precursors (ENPs). To analyze the function of <jats:italic>SEMA3A<\/jats:italic> in HSCR, the <jats:italic>SEMA3A<\/jats:italic> expression in different colon segments in HSCR was examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>The expression levels of <jats:italic>SEMA3A<\/jats:italic> in both ganglionic and aganglionic colon tissues of 32 patients with HSCR and in colon tissue of 5 newborn unaffected individuals were examined by real\u2010time RT\u2010PCR, Western\u2010blot, and immunohistology.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Comparison of <jats:italic>SEMA3A<\/jats:italic> expression levels between ganglionic and aganglionic tissues in HSCR revealed upregulation of <jats:italic>SEMA3A<\/jats:italic> expression in 43.75% (14\/32) of the aganglionic colons. <jats:italic>SEMA3A<\/jats:italic> was expressed in the ganglion cells of the myenteric plexus, submucosa, as well as in the longitudinal and circular muscle layer of the normal colon of both unaffected newborns and patients with HSCR. In the aganglionic segment of patients with HSCR, <jats:italic>SEMA3A<\/jats:italic> was highly expressed in the circular muscle layer and was also detected in the submucosa and in the longitudinal muscles layer. The fluorescence intensity of <jats:italic>SEMA3A<\/jats:italic> in the circular muscle layer in the aganglionic segment was much higher than that in ganglionic segment (<jats:italic>p<\/jats:italic> &lt; .001).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>SEMA3A expression was upregulated in the aganglionic smooth muscle layer of the colon in some patients with HSCR and our data suggest that increased <jats:italic>SEMA3A<\/jats:italic> expression may be a risk factor for HSCR pathology in a subset of patients. Birth Defects Research (Part A), 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdra.20837","type":"journal-article","created":{"date-parts":[[2011,6,8]],"date-time":"2011-06-08T19:36:26Z","timestamp":1307561786000},"page":"842-847","source":"Crossref","is-referenced-by-count":23,"title":["Semaphorin 3A expression in the colon of Hirschsprung disease"],"prefix":"10.1002","volume":"91","author":[{"given":"Li\u2010Li","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng\u2010hua","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Ning","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian\u2010Chu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng\u2010Wei","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,6,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.02.020"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0893-351"},{"key":"e_1_2_6_4_1","first-page":"353","article-title":"Abnormal distribution of sarcoglycan subcomplex in colonic smooth muscle cells of aganglionic bowel","volume":"25","author":"Arena S","year":"2010","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_5_1","unstructured":"ArnoldS GuyM KashukK et al.2008. 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