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In a recent study, we reported that a diet supplemented with 20\u2010fold higher FA than the recommended intake for rodents had adverse effects on embryonic mouse development at embryonic days (E)10.5 and 14.5. In this report, we examined developmental outcomes in E14.5 embryos after administering a diet supplemented with 10\u2010fold higher FA than recommended to pregnant mice with and without a mild deficiency of methylenetetrahydrofolate reductase (MTHFR).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Pregnant mice with or without a deficiency in MTHFR were fed a control diet (recommended FA intake of 2 mg\/kg diet for rodents) or an FA\u2010supplemented diet (FASD; 10\u2010fold higher than the recommended intake [20 mg\/kg diet]). At E14.5, mice were examined for embryonic loss and growth retardation, and hearts were assessed for defects and for ventricular wall thickness.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Maternal FA supplementation was associated with embryonic loss, embryonic delays, a higher incidence of ventricular septal defects, and thinner left and right ventricular walls, compared to mothers fed control diet.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Our work suggests that even moderately high levels of FA supplementation may adversely affect fetal mouse development. Additional studies are warranted to evaluate the impact of high folate intake in pregnant women. Birth Defects Research (Part A), 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdra.23092","type":"journal-article","created":{"date-parts":[[2012,11,2]],"date-time":"2012-11-02T09:09:54Z","timestamp":1351847394000},"page":"47-52","source":"Crossref","is-referenced-by-count":79,"title":["Moderately high intake of folic acid has a negative impact on mouse embryonic development"],"prefix":"10.1002","volume":"97","author":[{"given":"Leonie 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