{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:30:57Z","timestamp":1786977057318,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,9,6]],"date-time":"2013-09-06T00:00:00Z","timestamp":1378425600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2013,9]]},"abstract":"\n BACKGROUND<\/jats:title>\n \n Plac1<\/jats:italic>\n is an X\u2010linked gene essential for normal placental development.\n Plac1<\/jats:italic>\n ablation is associated with placentomegaly and intrauterine growth retardation. Additionally,\n Plac1<\/jats:italic>\n \u2010null mice exhibit reduced postnatal viability. This study was conducted as part of our ongoing examination of the postnatal phenotype associated with\n Plac1<\/jats:italic>\n ablation.\n <\/jats:p>\n <\/jats:sec>\n \n METHODS<\/jats:title>\n \n Plac1<\/jats:italic>\n was deleted in murine embryonic stem cells and bred against a C57BL\/6 background. Mutant mice were genotyped using a polymerase chain reaction\u2013based strategy. Embryonic\n Plac1<\/jats:italic>\n expression was assessed by quantitative real\u2010time polymerase chain reaction, in situ hybridization, and \u03b2\u2010galactosidase expression.\n <\/jats:p>\n <\/jats:sec>\n \n RESULTS<\/jats:title>\n \n A total of 20% of the surviving X\n m\u2010<\/jats:sup>\n Y (knockout) males as well as 10\u201315% of the X\n m\u2010<\/jats:sup>\n X females (mutant maternal allele) developed lethal hydrocephalus at 4\u20138 weeks. By contrast, XX\n p\u2010<\/jats:sup>\n hets (paternal null allele) did not.\n Plac1<\/jats:italic>\n mRNA expression was detected in the fetal brain that markedly decreased after birth.\n Plac1<\/jats:italic>\n mRNA expression localized to the hindbrain and lateral ventricles of wild\u2010type embryos. Additionally,\n Plac1<\/jats:italic>\n expression was observed throughout the fetus including the fetal lungs, kidney, intestine, liver, and heart. Embryonic\n Plac1<\/jats:italic>\n expression was paternally imprinted with partial escape of the paternal allele from complete inactivation. The Plac1 protein localized to the apical surface of the epithelial cells lining the developing airways of the lung and proximal renal tubules, consistent with its localization in the differentiated syncytiotrophoblast.\n <\/jats:p>\n <\/jats:sec>\n \n CONCLUSION<\/jats:title>\n \n Plac1<\/jats:italic>\n is expressed in the brain in a developmentally regulated manner and its absence is linked to increased risk for hydrocephalus. It is also widely expressed in fetal tissues expanding the existing paradigm to include direct tissue\u2010specific roles during development.\n Birth Defects Research (Part A) 97:571\u2013577, 2013<\/jats:italic>\n . \u00a9 2013 Wiley Periodicals, Inc.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/bdra.23171","type":"journal-article","created":{"date-parts":[[2013,9,6]],"date-time":"2013-09-06T11:31:05Z","timestamp":1378467065000},"page":"571-577","source":"Crossref","is-referenced-by-count":14,"title":["Plac1 (placenta\u2010specific 1) is widely expressed during fetal development and is associated with a lethal form of hydrocephalus"],"prefix":"10.1002","volume":"97","author":[{"given":"Xiaoyuan","family":"Kong","sequence":"first","affiliation":[{"name":"Department of Pediatrics University of South Florida Morsani College of Medicine Tampa Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne M.","family":"Jackman","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of South Florida Morsani College of Medicine Tampa Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Fant","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of South Florida Morsani College of Medicine Tampa Florida"},{"name":"Department of Obstetrics and Gynecology University of South Florida Morsani College of Medicine Tampa Florida"},{"name":"Department of Pathology and Cell Biology University of South Florida Morsani College of Medicine Tampa Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,9,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/72.2.246"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80853-9"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0004.1979.tb00848.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6302"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70017-X"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1197-346"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.36.189.486"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8628(20000501)92:1<40::AID-AJMG7>3.0.CO;2-R"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.23.1.23"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000071595"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/153.1.383"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2006.041673"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22062"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0893-331"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb802"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401600101"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1819.2000.00740.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.33913"},{"key":"e_1_2_6_20_1","first-page":"18","article-title":"PLAC1, 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