{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T20:53:56Z","timestamp":1789764836574,"version":"4.0.1"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,6,13]],"date-time":"2014-06-13T00:00:00Z","timestamp":1402617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001159","name":"Hawaii Community Foundation","doi-asserted-by":"publisher","award":["13ADVC-60316"],"award-info":[{"award-number":["13ADVC-60316"]}],"id":[{"id":"10.13039\/100001159","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Genetic variations affecting neural tube closure along the head result in malformations to the face and brain, posing a significant impact on health care costs and the quality of life. <jats:bold>Methods:<\/jats:bold> We have established a mouse line from a mutation that arose spontaneously in our wild\u2010type colony that we called <jats:italic>tuft<\/jats:italic>. <jats:italic>Tuft<\/jats:italic> mice have heritable midline craniofacial defects featuring an anterior lipomatous cephalocele. <jats:bold>Results:<\/jats:bold> Whole\u2010mount skeletal stains indicated that affected newborns had a broader interfrontal suture where the cephalocele emerged between the frontal bones. Mice with a cephalocele positioned near the rostrum also presented craniofacial malformations such as ocular hypertelorism and midfacial cleft of the nose. Gross and histological examination revealed that the lipomatous cephalocele originated as a fluid filled cyst no earlier than E14.5 while embryos with a midfacial cleft was evident during craniofacial development at E11.5. Histological sections of embryos with a midfacial cleft revealed the cephalic neuroectoderm remained proximal or fused to the frontonasal ectoderm about the closure site of the anterior neuropore, indicating a defect to neural tube closure. We found the neural folds along the rostrum of E9 to E10.5 embryos curled inward and failed to close as well as embryos with exencephaly and anencephaly at later stages. Whole\u2010mount in situ hybridization of anterior markers Fgf8 and Sonic hedgehog indicated closure of the rostral site was compromised in severe cases. <jats:bold>Conclusion:<\/jats:bold> We present a model demonstrating how anterior cranial cephaloceles are generated following a defect to neural tube closure and relevance to subsequent craniofacial morphogenesis in the <jats:italic>tuft<\/jats:italic> mouse. Birth Defects Research (Part A) 100:598\u2013607, 2014. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bdra.23264","type":"journal-article","created":{"date-parts":[[2014,6,13]],"date-time":"2014-06-13T14:57:30Z","timestamp":1402671450000},"page":"598-607","source":"Crossref","is-referenced-by-count":6,"title":["Midline craniofacial malformations with a lipomatous cephalocele are associated with insufficient closure of the neural tube in the <i>tuft<\/i> mouse"],"prefix":"10.1002","volume":"100","author":[{"given":"Keith S.K.","family":"Fong","sequence":"first","affiliation":[{"name":"Department of Anatomy, Biochemistry, and Physiology University of Hawaii, John A. Burns School of Medicine Honolulu Hawaii"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana A.T.","family":"Adachi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Biochemistry, and Physiology University of Hawaii, John A. 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