{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:37:24Z","timestamp":1777480644228,"version":"3.51.4"},"reference-count":72,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T00:00:00Z","timestamp":1450656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Supported by FAPESP (Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de S\u00e3o Paulo - The State of S\u00e3o Paulo Research Foundation","award":["2012\/10643-3"],"award-info":[{"award-number":["2012\/10643-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Alcohol exerts teratogenic effects and its consumption during pregnancy can cause deficit of bone development. The aim of the current study was to evaluate the genotoxic effects of prenatal exposure to ethanol on newborn rat osteoblasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Wistar rats were initially divided into two groups: Ethanol group which received Ethanol 20% V\/V in liquid diet and solid diet ad libitum, and Control group, which received solid diet and water ad libitum. Each group received a specific diet for 8 weeks before breeding and throughout three weeks of gestation and the treatment was finished on the day the pups were killed. On the fifth day of life, the pups from each group were killed for removal of the calvaria and isolation of osteogenic cells by sequential enzymatic digestion. The cells were cultured for a maximum period of 14 days. The detection of genotoxic effects of alcohol was investigated by the comet and the micronucleus assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Micronucleus and comet assay showed significant increases in DNA damage at 7 days in Ethanol group (<jats:italic>p<\/jats:italic>= 0.0302,<jats:italic>p<\/jats:italic>= 0.0446, respectively). However, at 14 days both assay showed no significant difference between the groups (<jats:italic>p<\/jats:italic>= 0.6194,<jats:italic>p<\/jats:italic>= 0.8326, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results showed that prenatal exposure to ethanol induced DNA damage in osteoblasts, as shown by micronucleus formation and higher percentage of DNA in the comet tail. It can be concluded that prenatal exposure to ethanol damages osteoblast DNA in newborns exposed to high doses of ethanol during pregnancy, suggesting that prenatal ethanol consumption has a direct effect on fetal osteoblasts. Birth Defects Research (Part A) 106:122\u2013132, 2016. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdra.23468","type":"journal-article","created":{"date-parts":[[2015,12,21]],"date-time":"2015-12-21T14:12:48Z","timestamp":1450707168000},"page":"122-132","source":"Crossref","is-referenced-by-count":12,"title":["High doses of alcohol during pregnancy cause DNA damages in osteoblasts of newborns rats"],"prefix":"10.1002","volume":"106","author":[{"given":"Isabel Chaves Silva","family":"Carvalho","sequence":"first","affiliation":[{"name":"Institute of Science and Technology, UNESP \u2013 Univ Estadual Paulista, Sao Jose dos Campos (SP), Department of Biosciences and Oral Diagnosis Sao Jose dos Campos Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamires Pereira","family":"Dutra","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology, UNESP \u2013 Univ Estadual Paulista, Sao Jose dos Campos (SP), Department of Biosciences and Oral Diagnosis Sao Jose dos Campos Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennia Perez","family":"De Andrade","sequence":"additional","affiliation":[{"name":"Institute of Research and Development \u2013 IP&amp;D, Universidade do Vale do Para\u00edba \u2013 UNIVAP, Laboratory Dynamics of Cellular Compartments Sao Jose dos Campos Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Balducci","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology, UNESP \u2013 Univ Estadual Paulista, Sao Jose dos Campos (SP), Department of Social Science and Pediatric Dentristy Sao Jose dos Campos Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Pacheco\u2010Soares","sequence":"additional","affiliation":[{"name":"Institute of Research and Development \u2013 IP&amp;D, Universidade do Vale do Para\u00edba \u2013 UNIVAP, Laboratory Dynamics of Cellular Compartments Sao Jose dos Campos Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosilene Fernandes da","family":"Rocha","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology, UNESP \u2013 Univ Estadual Paulista, Sao Jose dos Campos (SP), Department of Biosciences and Oral Diagnosis Sao Jose dos Campos Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,12,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2010.04.002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2001.tb02210.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-2333(00)00022-9"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/geq110"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1997.tb04256.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3844\/ojbsci.2015.134.142"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0740-5472(99)00105-1"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-6009(08)80221-8"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1385\/MB:26:3:249"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1542\/peds.84.3.536","article-title":"Prenatal exposure to alcohol: effect on infant growth and morphologic characteristics","volume":"84","author":"Day NL","year":"1989","journal-title":"Pediatrics"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2002.tb02459.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1563\/AAID-JOI-D-10-00048"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(98)00205-1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(94)00013-3"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(85)90169-9"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(00)00065-8"},{"key":"e_1_2_6_18_1","first-page":"1128","article-title":"Chromosomal biomarkers of genomic instability relevant to cancer","volume":"7","author":"Fenech M.","year":"2002","journal-title":"Drug Discov"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2003.tb02725.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(91)90044-W"},{"key":"e_1_2_6_21_1","first-page":"211","article-title":"Effect of prenatal ethanol exposure on the growth of rat mandible skeletal units","volume":"15","author":"Giglio MJ","year":"1987","journal-title":"J Biol Buccale"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572010005000118"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ALC.0000118315.58404.C1"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/18.2.201"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.12.020"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.23232"},{"key":"e_1_2_6_27_1","first-page":"31","article-title":"Preventing impaired driving","volume":"23","author":"Hingson RW","year":"1999","journal-title":"Alcohol Res Health"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1542\/peds.2004-0259","article-title":"A practical clinical approach to diagnosis of fetal alcohol spectrum disorders: clarification of the 1996 institute of medicine criteria","volume":"15","author":"Hoyme HE","year":"2005","journal-title":"Pediatrics"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1996.tb01127.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1997.tb04497.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ALC.0000086063.71754.C1"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ALC.0000118312.38204.C5"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1996.tb01095.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1997.tb03781.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgs340"},{"key":"e_1_2_6_36_1","first-page":"327","article-title":"Effect of litter size on the physical growth and maturation of the offspring of rats given alcohol during gestation","volume":"44","author":"Lee M","year":"1980","journal-title":"Growth"},{"key":"e_1_2_6_37_1","first-page":"254","article-title":"Skeletal development in fetuses of rats consuming alcohol during gestation","volume":"47","author":"Lee M","year":"1983","journal-title":"Growth"},{"key":"e_1_2_6_38_1","first-page":"288","article-title":"Effect of maternal ethanol administration on physical growth of the offspring in rats","volume":"43","author":"Leichter J","year":"1979","journal-title":"Growth"},{"key":"e_1_2_6_39_1","first-page":"84","article-title":"Alcohol and the liver: metabolism of alcohol and its role in hepatic and extrahepatic diseases","volume":"67","author":"Lieber CS","year":"2000","journal-title":"Mt Sinai J Med"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/15.6.517"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.2.270"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.04.011"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2014.01.008"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drugalcdep.2013.07.013"},{"key":"e_1_2_6_45_1","first-page":"3","article-title":"Nutrition and chronic alcohol abuse","volume":"23","author":"Moreno Otero R","year":"2008","journal-title":"Nutr Hosp"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2012.02.007"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0029(19960201)33:2<214::AID-JEMT11>3.0.CO;2-X"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.23142"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2007.10.007"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1177\/0883073811428376"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD11200"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2006.06.004"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.1271"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2004.12.010"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2002.tb02480.x"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2191"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2013.04.005"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572000000100042"},{"key":"e_1_2_6_59_1","first-page":"7","article-title":"Ethanol exposition in the perinatal to aguardente into rats cerebral cortex","volume":"20","author":"Silva BPF","year":"2006","journal-title":"Rev Para Med"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2004.11.011"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(88)90265-0"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1678-77572006000600010"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1806-83242009000400017"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1806-83242009000300017"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1000360206"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.2486\/indhealth.45.343"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5718(96)00140-4"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a116159"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2005.11.026"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/0741-8329(90)90020-D"},{"key":"e_1_2_6_71_1","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/S0022-3565(25)32467-5","article-title":"Interaction of ethanol and nutrition during gestation: influence on maternal and offspring development in the rat","volume":"216","author":"Wiener SG","year":"1981","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1590"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.23352"}],"container-title":["Birth Defects Research Part A: Clinical and Molecular Teratology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbdra.23468","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdra.23468","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T21:53:04Z","timestamp":1748728384000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdra.23468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,21]]},"references-count":72,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1002\/bdra.23468"],"URL":"https:\/\/doi.org\/10.1002\/bdra.23468","archive":["Portico"],"relation":{},"ISSN":["1542-0752","1542-0760"],"issn-type":[{"value":"1542-0752","type":"print"},{"value":"1542-0760","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,21]]}}}