{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T04:23:43Z","timestamp":1697084623972},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,8,26]],"date-time":"2003-08-26T00:00:00Z","timestamp":1061856000000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research Pt B"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND:<\/jats:bold> Microtia is a reduction in pinna size, usually seen in humans in conjunction with other medical conditions. We report microtia in CD\u20101 mice after gestational exposure to ethane dimethanesulfonate (EDS), an alkylating agent and adult rat Leydig cell toxicant. <jats:bold>METHODS:<\/jats:bold> Time\u2010pregnant CD\u20101 mice were administered 0, 80, or 160 mg EDS\/kg on gestation days (GD) 11\u201317, or 0 or 160 mg EDS\/kg on GD 11\u201313, GD 13\u201315 or GD 15\u201317. Pinnae were measured on postnatal days (PND) 4, 8, 18, and 28; and were observed for detachment from birth through PND 8. Branchial\u2010arch derived skeletal structures and histology of the pinna was examined on PND 4 and 24. Brainstem auditory evoked response (BAER) tests were carried out at approximately PND 160 to determine possible effects on hearing. <jats:bold>RESULTS:<\/jats:bold> All offspring of EDS\u2010treated dams exhibited bilateral, dose\u2010related decreases in pinna size. Gestational exposure during GD 11\u201313 produced smaller ears than during GD 13\u201315 or 15\u201317, but not as small as the GD 11\u201317 regimen. Ossification of other pharyngeal arch derivatives was delayed whereas histology was unremarkable. BAER analysis showed a decrease in the proportion of adult offspring producing a quantifiable response to varied auditory stimuli among EDS\u2010treated litters. <jats:bold>CONCLUSIONS:<\/jats:bold> Gestational exposure to EDS affects pinna development in the mouse, with a broad period of sensitivity during the second half of gestation. Microtia induced by EDS may be associated with hearing deficits, suggesting functional importance of pinna size or additional effects of EDS on ear development not detected by morphological examination. Birth Defects Res B68:383\u2013390, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/bdrb.10033","type":"journal-article","created":{"date-parts":[[2003,9,2]],"date-time":"2003-09-02T19:34:23Z","timestamp":1062531263000},"page":"383-390","source":"Crossref","is-referenced-by-count":2,"title":["Effects of gestational exposure to ethane dimethanesulfonate in CD\u20101 mice: Microtia and preliminary hearing tests"],"prefix":"10.1002","volume":"68","author":[{"given":"Dana K.","family":"Tarka\u2010Leeds","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David W.","family":"Herr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary R.","family":"Klinefelter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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