{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:10:00Z","timestamp":1760217000509},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,13]],"date-time":"2005-06-13T00:00:00Z","timestamp":1118620800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research Pt B"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>BACKGROUND:<\/jats:bold> Ethylnitrosourea (ENU), a monofunctional alkylating agent, induces apoptosis and cell cycle arrest in neuroepithelial cells, neural stem cells in the fetal central nervous system (CNS). These effects occur immediately after the administration of ENU to pregnant animals resulting in fetal brain anomalies and long\u2010term effects include brain tumors in the offspring. <jats:bold>METHODS:<\/jats:bold> Changes in gene expression were investigated in the fetal CNS after ENU administration to pregnant rats using microarray to identify the genes involved in the injury and recovery of the fetal CNS. <jats:bold>RESULTS:<\/jats:bold> The up\u2010regulation of 21 genes in injury and 15 genes in recovery phases and down\u2010regulation of 5 genes in injury and 3 genes in recovery phases were identified. The genes up\u2010regulated in the injury phase contained p53\u2010target genes that mediate apoptosis and cell cycle arrest, and those in the recovery phase contained cell proliferation\u2010promoting genes. The genes down\u2010regulated in the injury phase contained cholesterol biosynthesis\u2010related genes. In addition, there were some genes that have not been identified to be involved in the CNS injury and recovery. <jats:bold>CONCLUSIONS:<\/jats:bold> The present study will provide a better understanding of the mechanisms of development, regeneration and carcinogenesis of the CNS as well as the mechanisms of ENU\u2010induced fetal CNS injury and recovery. <jats:italic>Birth Defects Res B<\/jats:italic> 74:255\u2013260, 2005. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/bdrb.20045","type":"journal-article","created":{"date-parts":[[2005,6,13]],"date-time":"2005-06-13T15:46:56Z","timestamp":1118677616000},"page":"255-260","source":"Crossref","is-referenced-by-count":6,"title":["Microarray analysis of genes in fetal central nervous system after ethylnitrosourea 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