{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T05:32:00Z","timestamp":1781587920969,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,2,6]],"date-time":"2012-02-06T00:00:00Z","timestamp":1328486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research Pt B"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>The notochord has important structural and signaling properties during vertebrate development with key roles in patterning surrounding tissues, including the foregut. The adriamycin mouse model is an established model of foregut anomalies where exposure of embryos in utero to the drug adriamycin leads to malformations including oesophageal atresia and tracheoesophageal fistula. In addition to foregut abnormalities, treatment also causes branching, displacement, and hypertrophy of the notochord. Here, we explore the hypothesis that the notochord may be a primary target of disruption leading to abnormal patterning of the foregut by examining notochord position and structure in early embryos following adriamycin exposure. Treated (<jats:italic>n<\/jats:italic> = 46) and control (<jats:italic>n<\/jats:italic> = 30) embryos were examined during the crucial period when the notochord normally delaminates away from the foregut endoderm (6\u201328 somite pairs). Transverse sections were derived from the anterior foregut and analyzed by confocal microscopy following immunodetection of extracellular matrix markers <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>\u2010cadherin and Laminin. In adriamycin\u2010treated embryos across all stages, the notochord was abnormally displaced ventrally with prolonged attachment to the foregut endoderm. While <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>\u2010cadherin was normally detected in the foregut endoderm with no expression in the notochord of control embryos, treated embryos up to 24 somites showed ectopic notochordal expression indicating a change in characteristics of the tissue; specifically an increase in intracellular adhesiveness, which may be instrumental in structural changes, affecting mechanical and signaling properties. This is consistent with disruption of the notochord leading to altered signaling to the foregut causing abnormal patterning and congenital foregut malformations.<\/jats:p>","DOI":"10.1002\/bdrb.21002","type":"journal-article","created":{"date-parts":[[2012,2,6]],"date-time":"2012-02-06T14:52:30Z","timestamp":1328539950000},"page":"175-183","source":"Crossref","is-referenced-by-count":14,"title":["The Effect of Adriamycin Exposure on the Notochord of Mouse Embryos"],"prefix":"10.1002","volume":"95","author":[{"given":"Piotr","family":"Hajduk","sequence":"first","affiliation":[{"name":"School of Natural Sciences Trinity College Dublin  Dublin Ireland"},{"name":"National Children's Research Centre Our Lady's Children's Hospital  Crumlin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"May","sequence":"additional","affiliation":[{"name":"School of Natural Sciences Trinity College Dublin  Dublin Ireland"},{"name":"National Children's Research Centre Our Lady's Children's Hospital  Crumlin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prem","family":"Puri","sequence":"additional","affiliation":[{"name":"National Children's Research Centre Our Lady's Children's Hospital  Crumlin Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Murphy","sequence":"additional","affiliation":[{"name":"School of Natural Sciences Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,2,6]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19960616)61:4<514::AID-JCB4>3.0.CO;2-R"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1242\/dev.110.1.115","article-title":"The mechanics of notochord elongation, straightening and stiffening in the embryo of \n                     Xenopus laevis","volume":"110","author":"Adams DS","year":"1990","journal-title":"Development"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0214"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2004.07.020"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-006-1847-9"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2006.10.011"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11894-010-0108-1"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2003.50081"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027635"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.041095"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90069-8"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.8.985"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176009"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2002.30252"},{"key":"e_1_2_6_16_1","first-page":"1","article-title":"Some aspects of the development of the notochord in mouse embryos","volume":"32","author":"Jurand A","year":"1974","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(93)90250-L"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12565-009-0020-7"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.17.8263"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.20.010403.113126"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.12.010"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2004.10.056"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.6.2649"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9047"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1053\/jpsu.2001.24722"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.13.3137"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003830050555"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3468(99)90623-1"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.2006.00096.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/383627a0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-2-24"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2006419"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88418-4"},{"key":"e_1_2_6_34_1","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/s00383-002-0887-z","article-title":"Long\u2010term evaluation of esophageal function in patients treated at birth for esophageal atresia","volume":"19","author":"Tomaselli V","year":"2003","journal-title":"Pediatr Surg Int"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2009.08.003"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.033910"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003830000440"}],"container-title":["Birth Defects Research Part B: Developmental and Reproductive Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbdrb.21002","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdrb.21002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T06:34:19Z","timestamp":1698734059000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdrb.21002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,6]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1002\/bdrb.21002"],"URL":"https:\/\/doi.org\/10.1002\/bdrb.21002","archive":["Portico"],"relation":{},"ISSN":["1542-9733","1542-9741"],"issn-type":[{"value":"1542-9733","type":"print"},{"value":"1542-9741","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,6]]}}}