{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:28:30Z","timestamp":1760549310668},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T00:00:00Z","timestamp":1358985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research Pt B"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>This study was conducted as part of an <jats:styled-content style=\"fixed-case\">ILSI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HESI<\/jats:styled-content> International Life Sciences Institute\u2010Health &amp; Environmental Sciences Institute consortium effort to assess the utility of circulating Inhibin B as an early biomarker of Sertoli cell\u2010specific testicular toxicity in rats. 1, 3\u2010Dinitrobenzene (1,3\u2010DNB) was selected as a testicular toxicant in this study as it is known to target Sertoli cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>1,3\u2010DNB (2 and 6 mg\/kg\/day) or control (corn oil) was administered orally to male rats for two or five consecutive days. Blood was collected from rats treated for 2 days on days 1 and 2 and from rats treated for 5 days on days 1, 3, and 5. The resulting serum was evaluated for Inhibin B and follicle stimulating hormone. At the end of the treatment periods, the testes were removed, weighed, and examined histopathologically.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Daily administration of 1,3\u2010DNB resulted in decreased testis weight only on day 5 and only at the high dose (6 mg\/kg\/day). There was a time\u2010dependent increase in incidence and severity of testicular findings characterized by degeneration of the germinal epithelium with loss of pachytene spermatocytes and vacuolization of the Sertoli cells in the seminiferous tubules at the high dose. Inhibin B levels in 1,3\u2010DNB\u2010treated animals were decreased with treatment only on day 5 at the high dose; there were no associated changes in follicle stimulating hormone.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Changes in serum Inhibin B levels were detected only in association with moderate or severe testicular toxicity as evidenced by histopathology and is therefore considered to be of limited value as a biomarker for Sertoli cell toxicity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdrb.21038","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T17:36:17Z","timestamp":1359048977000},"page":"29-34","source":"Crossref","is-referenced-by-count":4,"title":["The Inhibin B Response to the Testicular Toxicant 1, 3 Dinitrobenzene in Male Rats"],"prefix":"10.1002","volume":"98","author":[{"given":"Manisha","family":"Sonee","sequence":"first","affiliation":[{"name":"Drug Safety Sciences Janssen Research &amp; Development LLC Raritan  New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Bogdan","sequence":"additional","affiliation":[{"name":"Drug Safety Sciences Janssen Research &amp; Development LLC Raritan  New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LeRoy","family":"Hall","sequence":"additional","affiliation":[{"name":"Drug Safety Sciences Janssen Research &amp; Development LLC Raritan  New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stewart","family":"Bryant","sequence":"additional","affiliation":[{"name":"Drug Safety Sciences Janssen Research &amp; Development LLC Raritan  New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Vinken","sequence":"additional","affiliation":[{"name":"Drug Safety Sciences Janssen Research &amp; Development, a Division of Janssen Pharmaceutica NV  Beerse Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.81.9.3341"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(01)00510-X"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(88)90227-X"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287398109530024"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq210"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1988.tb01059.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.25.SpecialIssue_251"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2004-831895"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2012.07.010"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385961-7.00014-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1450561"},{"issue":"9","key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"3110","DOI":"10.1210\/jcem.83.9.5121","article-title":"Serum Inhibin B as a marker of spermatogenesis","volume":"83","author":"Pierik FH","year":"1998","journal-title":"J Clinical Endocrinol"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0272-0590(91)90135-Q"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-2005-1109"}],"container-title":["Birth Defects Research Part B: Developmental and Reproductive Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbdrb.21038","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdrb.21038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T11:50:59Z","timestamp":1694519459000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdrb.21038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,24]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1002\/bdrb.21038"],"URL":"https:\/\/doi.org\/10.1002\/bdrb.21038","archive":["Portico"],"relation":{},"ISSN":["1542-9733","1542-9741"],"issn-type":[{"value":"1542-9733","type":"print"},{"value":"1542-9741","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,24]]}}}