{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T01:42:54Z","timestamp":1759542174307},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T00:00:00Z","timestamp":1358985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research Pt B"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:sec><jats:title>OBJECTIVE<\/jats:title><jats:p>The objective of this study was to evaluate the utility of plasma Inhibin B (InhB) as a biomarker of testicular injury in adult rats following short\u2010term exposure to the known Sertoli cell toxicants mono\u20102\u2010ethylhexyl phthalate (<jats:styled-content style=\"fixed-case\">MEHP<\/jats:styled-content>), 1,3 dinitrobenzene (<jats:styled-content style=\"fixed-case\">DNB<\/jats:styled-content>), or carbendazim (<jats:styled-content style=\"fixed-case\">CBZ<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Following oral gavage administration of the compounds for 2 or 7 days, the rats were evaluated for clinical signs, body weight, food consumption, organ weights, plasma hormone levels, and gross and microscopic pathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MEHP<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">DNB<\/jats:styled-content>, and<jats:styled-content style=\"fixed-case\">CBZ<\/jats:styled-content>produced a range of testicular toxicity characterized by minimal exfoliation of germ cells as demonstrated by increased cellular debris in the epididymis (<jats:styled-content style=\"fixed-case\">MEHP<\/jats:styled-content>) to more severe and dose\/duration responsive Sertoli cell vacuolation, germ cell degeneration, and multinucleated giant cells of germ cell origin (<jats:styled-content style=\"fixed-case\">DNB<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">CBZ<\/jats:styled-content>). The slight to moderate Sertoli and germinal cell injuries did not correlate with significant changes in plasma InhB levels following 2\u2010 or 7\u2010day exposures. However, more severe injury to germinal epithelium following up to 7 days of exposure, but not after 2 days of exposure, correlated with decreased plasma InhB levels and less consistently with increases in plasma follicle stimulating hormone.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>In conclusion, under the conditions of these studies, changes in InhB were not an effective early onset marker of testicular toxicity or an effective marker for slight to moderate levels of acute injury, and only reflected more severe disruption of spermatogenesis. Changes in plasma InhB and follicle stimulating hormone were poorly correlated except in some instances of moderate to marked testicular toxicity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdrb.21043","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T17:37:16Z","timestamp":1359049036000},"page":"72-81","source":"Crossref","is-referenced-by-count":9,"title":["The Inhibin B (<scp>I<\/scp>nh<scp>B<\/scp>) Response to the Testicular Toxicants Mono\u20102\u2010Ethylhexyl Phthalate (<scp>MEHP<\/scp>), 1,3 Dinitrobenzene (<scp>DNB<\/scp>), or Carbendazim (<scp>CBZ<\/scp>) Following Short\u2010term Repeat Dosing in the Male Rat"],"prefix":"10.1002","volume":"98","author":[{"given":"William J.","family":"Breslin","sequence":"first","affiliation":[{"name":"Non\u2010Clinical Safety Assessment, Lilly Research Laboratories Lilly Corporate Center Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"April","family":"Paulman","sequence":"additional","affiliation":[{"name":"Department of Pathology Covance Laboratories Inc Greenfield Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise","family":"Sun\u2010Lin","sequence":"additional","affiliation":[{"name":"Investigative Toxicology, Lilly Research Laboratories Lilly Corporate Center Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith M.","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Investigative Toxicology, Lilly Research Laboratories Lilly Corporate Center Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Derr","sequence":"additional","affiliation":[{"name":"Discovery and Translational Services Covance Laboratories Inc Greenfield Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(89)90305-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/03602538909029953"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(01)00510-X"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(88)90227-X"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"14.4.1","DOI":"10.1002\/0471140856.tx1604s13","article-title":"Histopathology of the male reproductive system II, interpretation","volume":"13","author":"Creasy DM","year":"2002","journal-title":"Curr Protoc Toxicol"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(01)00348-4"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2307\/2528490"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq210"},{"key":"e_1_2_6_10_1","first-page":"443","volume-title":"Fundamentals of toxicologic pathology","author":"Haschek WM","year":"1998"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2131\/jts.25.SpecialIssue_251"},{"key":"e_1_2_6_12_1","first-page":"278","volume-title":"Contributions to probability and statistics","author":"Levene H","year":"1960"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1996.8042"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385961-7.00014-7"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1450561"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/01926230701481931"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/52.3-4.591"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.2550090311"},{"key":"e_1_2_6_19_1","volume-title":"Memorandum. 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