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The effect of ethanol has been reported to affect cellular development on the embryonic level, however, not much is known about mutations contributing to the influence of ethanol. The purpose of our study was to determine if mutation contribute to changes in differentiation patterning, cell\u2010cycle regulatory gene expression, and DNA methylation in human embryonic stem cells after ethanol exposure. We exposed human embryonic stem cells (with and without know DNA mutations) to a low concentration (20 mM) of ethanol and measured neurosphere proliferation and differentiation, glial protein levels, expression of various cell\u2010cycle genes, and DNA methylation. Ethanol altered cell\u2010cycle gene expression between the two cell lines; however, gene methylation was not affected in ether lines.. Birth Defects Res (Part B) 98:283\u2013295, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bdrb.21063","type":"journal-article","created":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T20:39:32Z","timestamp":1369168772000},"page":"283-295","source":"Crossref","is-referenced-by-count":15,"title":["Ethanol Alters Proliferation and Differentiation of Normal and Chromosomally Abnormal Human Embryonic Stem Cell\u2010Derived Neurospheres"],"prefix":"10.1002","volume":"98","author":[{"given":"Malini","family":"Krishnamoorthy","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Emory University School of Medicine Atlanta Georgia"},{"name":"Jeevan Biosciences INC. 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