{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T08:03:15Z","timestamp":1745049795952},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,1,9]],"date-time":"2014-01-09T00:00:00Z","timestamp":1389225600000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research Pt B"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:p>The kidney is one of the key organs in clearing foreign compounds. The effects of drugs on the developing kidney are relatively unknown. We studied the direct effect of furosemide, hydrochlorothiazide, ibuprofen, and indomethacin on kidney development in an ex vivo embryonic kidney model. At embryonic day 13, metanephroi were dissected from mice and cultured in control media or media supplemented with various clinically relevant concentrations of drugs. The ureteric tree was visualized by whole\u2010mount staining and branching was evaluated by counting. Additionally, gene expression levels of <jats:italic>Wt1<\/jats:italic>, <jats:italic>Sox9<\/jats:italic>, <jats:italic>Bmp7<\/jats:italic>, <jats:italic>Fgf8<\/jats:italic>, and <jats:italic>Gdnf<\/jats:italic> were investigated. No distinct differences were noted on either ureteric tip development or gene expression analysis for each drug after 24 hr of exposure. Even though short\u2010term exposure to clinically relevant concentrations seems not to disturb renal development, future research is needed to study prolonged or repeated exposures.<\/jats:p>","DOI":"10.1002\/bdrb.21090","type":"journal-article","created":{"date-parts":[[2014,1,10]],"date-time":"2014-01-10T00:54:40Z","timestamp":1389315280000},"page":"486-492","source":"Crossref","is-referenced-by-count":5,"title":["Effect of NSAIDs and Diuretics on Nephrogenesis in an Ex Vivo Embryogenic Kidney Model"],"prefix":"10.1002","volume":"98","author":[{"given":"Ruud RG","family":"Bueters","sequence":"first","affiliation":[{"name":"Department of Pediatric Nephrology Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annelies","family":"Klaasen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology Radboud University Medical Center  Nijmegen The 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