{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T02:43:50Z","timestamp":1773283430232,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T00:00:00Z","timestamp":1442361600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Kolff","award":["M.F.S. (KJPB.08.06)"],"award-info":[{"award-number":["M.F.S. (KJPB.08.06)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Birth Defects Research Pt B"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Prematurely born children with patent ductus arteriosus are treated with ibuprofen or indomethacin, which may inhibit kidney development. We determined whether clinical doses affected kidney development and function, with or without extrauterine growth retardation.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Wistar rats were cross\u2010fostered in normal food (NF) or food restricted (FR) litters at postnatal day (PND) 2. On PND 3 to 4, three doses of 0.9% NaCl, 0.1 mg\/kg indomethacin, or 10 mg\/kg ibuprofen were administered via intraperitoneal injection with 12\u2010hr intervals. Kidneys were evaluated for apoptosis, proliferation, and gene expression at PND 8; stereological assessment of nephron number at PND 35; and clinical pathology and neutrophil gelatinase\u2010associated lipocalin at 4 and 9 months. Blood pressure was measured at the ages of 4, 6, and 9 months.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>NF and FR bodyweight differed from PND 3 onwards, ranging from 16.5 g at weaning (<jats:italic>p<\/jats:italic> &lt; 0.001) to 39 g at necropsy (<jats:italic>p<\/jats:italic> = 0.019). Kidney proliferation\/apoptosis ratios were 7:1 and 3:1 (<jats:italic>p<\/jats:italic> = 0.001), respectively and different expression of <jats:italic>Wnt4<\/jats:italic> (0.7x<jats:italic>), Oat1<\/jats:italic> (1.3x), <jats:italic>Nphs1<\/jats:italic> (1.7x), <jats:italic>and Aqp4<\/jats:italic> (1.3x) was noted (but its biological relevance doubted). Nephron numbers were decreased by 12% (<jats:italic>p<\/jats:italic> = 0.109) in the ibuprofen\u2010NF group and 7.5% (<jats:italic>p<\/jats:italic> = 0.237) in FR groups. This coincided with a tendency to increased neutrophil gelatinase\u2010associated lipocalin at 9 months. No differences were noted in electrolytes, creatinine, or urea clearance. No valid blood pressure results could be obtained.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>A clinical Ibuprofen dose showed potential to inhibit kidney development in neonatal rats. FR did not modulate these effects<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdrb.21161","type":"journal-article","created":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T20:45:04Z","timestamp":1442436304000},"page":"218-226","source":"Crossref","is-referenced-by-count":9,"title":["Impact of Early Postnatal NSAID Treatment on Nephrogenesis in Wistar Rats"],"prefix":"10.1002","volume":"104","author":[{"given":"Ruud R. G.","family":"Bueters","sequence":"first","affiliation":[{"name":"Department of Pediatric Nephrology Radboud University Medical Center  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annelies","family":"Klaasen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology Radboud University Medical Center  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuria","family":"Maicas","sequence":"additional","affiliation":[{"name":"Department of Experimental Nephrology Radboud University Medical Center  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Florquin","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lambertus P.","family":"van den Heuvel","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology Radboud University Medical Center  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiel F.","family":"Schreuder","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology Radboud University Medical Center  Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-003-1402-z"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.4103\/1117-1936.171631","article-title":"The effect of the cyclooxygenase blockers, ibuprofen on the development of glomeruli in Sprague\u2010Dawley rats","volume":"10","author":"Akinola O","year":"2003","journal-title":"Niger Postgrad Med J"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2009.201"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/14767058.2012.733775"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-011-1843-8"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.03530708"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.265"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2004.tb00844.x"},{"issue":"80","key":"e_1_2_5_10_1","first-page":"1","article-title":"Early postnatal low\u2010protein nutrition, metabolic programming and the autonomic nervous system in adult life","volume":"9","author":"Oliveira JC","year":"2012","journal-title":"Nutr Metab (Lond)"},{"issue":"7","key":"e_1_2_5_11_1","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1096\/fasebj.2.7.3280378","article-title":"Age\u2010related nephropathy in laboratory rats","volume":"2","author":"Goldstein RS","year":"1988","journal-title":"FASEB J"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00380-012-0273-2"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(11)80177-9"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000081467"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2005-0309"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e318123f6e3"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-008-1114-5"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000088027.54400.C6"},{"key":"e_1_2_5_19_1","first-page":"CD003481","article-title":"Ibuprofen for the treatment of patent ductus arteriosus in preterm and\/or low birth weight infants","volume":"4","author":"Ohlsson A","year":"2013","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2290"},{"key":"e_1_2_5_21_1","first-page":"3","volume-title":"Brenner and Rector's the kidney","author":"Quaggin SE","year":"2008"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10024-003-3029-2"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-012-2169-x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00158.2006"},{"issue":"6","key":"e_1_2_5_25_1","first-page":"624","article-title":"The laboratory rat: relating its age with human's","volume":"4","author":"Sengupta P","year":"2013","journal-title":"Int J Prev Med"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200202000-00013"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2010121266"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00216.2011"}],"container-title":["Birth Defects Research Part B: Developmental and Reproductive Toxicology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbdrb.21161","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbdrb.21161","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdrb.21161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T18:48:14Z","timestamp":1696618094000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdrb.21161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,16]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1002\/bdrb.21161"],"URL":"https:\/\/doi.org\/10.1002\/bdrb.21161","archive":["Portico"],"relation":{},"ISSN":["1542-9733","1542-9741"],"issn-type":[{"value":"1542-9733","type":"print"},{"value":"1542-9741","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,16]]}}}