{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T13:03:22Z","timestamp":1772802202346,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,9,26]],"date-time":"2006-09-26T00:00:00Z","timestamp":1159228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioelectromagnetics"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An in vitro study focusing on the effects of low\u2010level radiofrequency (RF) fields from mobile radio base stations employing the International Mobile Telecommunication 2000 (IMT\u20102000) cellular system was conducted to test the hypothesis that modulated RF fields act to induce phosphorylation and overexpression of heat shock protein hsp27. First, we evaluated the responses of human cells to microwave exposure at a specific absorption rate (SAR) of 80 mW\/kg, which corresponds to the limit of the average whole\u2010body SAR for general public exposure defined as a basic restriction in the International Commission on Non\u2010Ionizing Radiation Protection (ICNIRP) guidelines. Second, we investigated whether continuous wave (CW) and Wideband Code Division Multiple Access (W\u2010CDMA) modulated signal RF fields at 2.1425 GHz induced activation or gene expression of hsp27 and other heat shock proteins (hsps). Human glioblastoma A172 cells were exposed to W\u2010CDMA radiation at SARs of 80 and 800 mW\/kg for 2\u201348 h, and CW radiation at 80 mW\/kg for 24 h. Human IMR\u201090 fibroblasts from fetal lungs were exposed to W\u2010CDMA at 80 and 800 mW\/kg for 2 or 28 h, and CW at 80 mW\/kg for 28 h. Under the RF field exposure conditions described above, no significant differences in the expression levels of phosphorylated hsp27 at serine 82 (hsp27[pS82]) were observed between the test groups exposed to W\u2010CDMA or CW signal and the sham\u2010exposed negative controls, as evaluated immediately after the exposure periods by bead\u2010based multiplex assays. Moreover, no noticeable differences in the gene expression of hsps were observed between the test groups and the negative controls by DNA Chip analysis. Our results confirm that exposure to low\u2010level RF field up to 800 mW\/kg does not induce phosphorylation of hsp27 or expression of hsp gene family. Bioelectromagnetics \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/bem.20277","type":"journal-article","created":{"date-parts":[[2006,9,26]],"date-time":"2006-09-26T18:27:54Z","timestamp":1159295274000},"page":"99-108","source":"Crossref","is-referenced-by-count":29,"title":["Mobile phone base station\u2010emitted radiation does not induce phosphorylation of Hsp27"],"prefix":"10.1002","volume":"28","author":[{"given":"H.","family":"Hirose","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Sakuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Kaji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Sekijima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Nojima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Miyakoshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.3.1276"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.8.12.5059"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009234200"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(99)00069-1"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.2001.670401.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.3.357"},{"key":"e_1_2_1_9_1","first-page":"273","article-title":"HSP27 phosphorylation\u2010mediated resistance against actin fragmentation and cell death induced by oxidative stress","volume":"56","author":"Huot J","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_1_10_1","first-page":"494","article-title":"Guidelines for limiting exposure to time varying electric, magnetic and electromagnetic fields (up to 300 GHz)","volume":"74","author":"ICNIRP","year":"1998","journal-title":"Health Phys"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/bem.20038"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.10.1.133-139.2003"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)78121-6"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01267.2001"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)48354-8"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.1.505"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.2002.700207.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/bem.20077"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.841339"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-2-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bem.20179"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00458.2003"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)81216-9"}],"container-title":["Bioelectromagnetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbem.20277","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bem.20277","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T17:24:35Z","timestamp":1695921875000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bem.20277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,26]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1002\/bem.20277"],"URL":"https:\/\/doi.org\/10.1002\/bem.20277","archive":["Portico"],"relation":{},"ISSN":["0197-8462","1521-186X"],"issn-type":[{"value":"0197-8462","type":"print"},{"value":"1521-186X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9,26]]}}}