{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T17:35:14Z","timestamp":1759772114564},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,10,30]],"date-time":"2009-10-30T00:00:00Z","timestamp":1256860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioelectromagnetics"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The growth of estrogen\u2010receptor positive breast cancer cells is inhibited by the pineal gland hormone, melatonin. Concern has been raised that power\u2010line frequency and microwave electromagnetic fields (EMFs) could reduce the efficiency of melatonin on breast cancer cells. In this study we investigated the impact of EMFs on the signal transduction of the high\u2010affinity receptor MT1 in parental MCF\u20107 cells and MCF\u20107 cells transfected with the MT1 gene. The binding of the cAMP\u2010responsive element binding (CREB) protein to a promoter sequence of BRCA\u20101 after stimulation with melatonin was analyzed by a gel\u2010shift assay and the expression of four estrogen\u2010responsive genes was measured in sham\u2010exposed breast cancer cells and cells exposed to a sinusoidal 50\u2009Hz EMF of 1.2\u2009\u00b5T for 48\u2009h. In sham\u2010exposed cells, binding of CREB to the promoter of BRCA\u20101 was increased by estradiol and subsequently diminished by treatment with melatonin. In cells exposed to 1.2\u2009\u00b5T, 50\u2009Hz EMF, binding of CREB was almost completely omitted. Expression of BRCA\u20101, p53, p21<jats:sup>WAF<\/jats:sup>, and c\u2010myc was increased by estradiol stimulation and subsequently decreased by melatonin treatment in both cell lines, except for p53 expression in the transfected cell line, thereby proving the antiestrogenic effect of melatonin at molecular level. In contrast, in breast cancer cells transfected with MT1 exposed to 1.2\u2009\u00b5T of the 50\u2009Hz EMF, the expression of p53 and c\u2010myc increased significantly after melatonin treatment but for p21<jats:sup>WAF<\/jats:sup> the increase was not significant. These results convincingly prove the negative effect of EMF on the antiestrogenic effect of melatonin in breast cancer cells. Bioelectromagnetics 31:237\u2013245, 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/bem.20554","type":"journal-article","created":{"date-parts":[[2009,10,30]],"date-time":"2009-10-30T14:32:28Z","timestamp":1256913148000},"page":"237-245","source":"Crossref","is-referenced-by-count":17,"title":["Signal transduction of the melatonin receptor MT1 is disrupted in breast cancer cells by electromagnetic 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