{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:36:23Z","timestamp":1770971783339,"version":"3.50.1"},"reference-count":63,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,4,9]],"date-time":"2012-04-09T00:00:00Z","timestamp":1333929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioelectromagnetics"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The potential health hazard of exposure to electromagnetic fields (EMF) continues to cause public concern. However, the possibility of biological and health effects of exposure to EMF remains controversial and their biophysical mechanisms are unknown. In the present study, we used <jats:italic>Saccharomyces cerevisiae<\/jats:italic> to identify genes responding to extremely low frequency magnetic fields (ELF\u2010MF) and to radiofrequency EMF (RF\u2010EMF) exposures. The yeast cells were exposed for 6\u2009h to either 0.4\u2009mT 50\u2009Hz ELF\u2010MF or 1800\u2009MHz RF\u2010EMF at a specific absorption rate of 4.7\u2009W\/kg. Gene expression was analyzed by microarray screening and confirmed using real\u2010time reverse transcription\u2010polymerase chain reaction (RT\u2010PCR). We were unable to confirm microarray\u2010detected changes in three of the ELF\u2010MF responsive candidate genes using RT\u2010PCR (<jats:italic>P<\/jats:italic>\u2009&gt;\u20090.05). On the other hand, out of the 40 potential RF\u2010EMF responsive genes, only the expressions of structural maintenance of chromosomes 3 (SMC3) and aquaporin 2 (AQY2 (m)) were confirmed, while three other genes, that is, halotolerance protein 9 (HAL9), yet another kinase 1 (YAK1) and one function\u2010unknown gene (open reading frame: YJL171C), showed opposite changes in expression compared to the microarray data (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). In conclusion, the results of this study suggest that the yeast cells did not alter gene expression in response to 50\u2009Hz ELF\u2010MF and that the response to RF\u2010EMF is limited to only a very small number of genes. The possible biological consequences of the gene expression changes induced by RF\u2010EMF await further investigation. Bioelectromagnetics  33:550\u2013560, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bem.21724","type":"journal-article","created":{"date-parts":[[2012,4,9]],"date-time":"2012-04-09T15:19:08Z","timestamp":1333984748000},"page":"550-560","source":"Crossref","is-referenced-by-count":23,"title":["Using model organism <i>Saccharomyces cerevisiae<\/i> to evaluate the effects of ELF\u2010MF and RF\u2010EMF exposure on global gene 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