{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T18:10:04Z","timestamp":1743271804260},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,3,15]],"date-time":"2016-03-15T00:00:00Z","timestamp":1458000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Ministry of Health","award":["RF-2009-1543811"],"award-info":[{"award-number":["RF-2009-1543811"]}]},{"name":"Catholic University (D.3.2 and D.1 funds)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioelectromagnetics"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:label \/><jats:p>This study investigates effects of gradient magnetic fields (GMFs) emitted by magnetic resonance imaging (MRI) devices on hematopoietic stem cells. Field measurements were performed to assess exposure to GMFs of staff working at 1.5 T and 3 T MRI units. Then an exposure system reproducing measured signals was realized to expose in vitro CD34+ cells to GMFs (1.5 T\u2010protocol and 3 T\u2010protocol). CD34+ cells were obtained by Fluorescence Activated Cell Sorting from six blood donors and three MRI\u2010exposed workers. Blood donor CD34+ cells were exposed in vitro for 72\u2009h to 1.5 T or 3 T\u2010protocol and to sham procedure. Cells were then cultured and evaluated in colony forming unit (CFU)\u2010assay up to 4 weeks after exposure. Results showed that in vitro GMF exposure did not affect cell proliferation but instead induced expansion of erythroid and monocytes progenitors soon after exposure and for the subsequent 3 weeks. No decrease of other clonogenic cell output (i.e., CFU\u2010granulocyte\/erythroid\/macrophage\/megakaryocyte and CFU\u2010granulocyte\/macrophage) was noticed, nor exposed CD34+ cells underwent the premature exhaustion of their clonogenic potential compared to sham\u2010exposed controls. On the other hand, pilot experiments showed that CD34+ cells exposed in vivo to GMFs (i.e., samples from MRI workers) behaved in culture similarly to sham\u2010exposed CD34+ cells, suggesting that other cells and\/or microenvironment factors might prevent GMF effects on hematopoietic stem cells in vivo. Accordingly, GMFs did not affect the clonogenic potential of umbilical cord blood CD34+ cells exposed in vitro together with the whole mononuclear cell fraction. 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