{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:12:43Z","timestamp":1791389563936,"version":"4.3.3"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T00:00:00Z","timestamp":1540771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008903","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["HN14C0086"],"award-info":[{"award-number":["HN14C0086"]}],"id":[{"id":"10.13039\/100008903","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioelectromagnetics"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>Melanocytes in hair are located around dermal papilla cells at the tip of the hair follicle. In this study, we examined the melanogenesis of a three\u2010dimensional (3D) hair dermal papilla model treated with natural extracts and electromagnetic fields (EMFs). The 3D model involved dermal papilla\u2010like tissue (DPLT), an aggregation of a mixture of dermal papilla cells, and melanocytes in microwells. Rice bran extract (RBE), an EMF, and RBE\/EMF were applied to different DPLT groups. The LDH assay indicated no cell stress in all experimental groups, and detection of tyrosinase activity demonstrated high activity in the RBE\/EMF group. Western blot analysis of the RBE, EMF, and RBE\/EMF groups revealed increased MITF, TRP\u20101, and tyrosinase expression. In addition, the mRNA expression of ET\u20101, laminin, bFGF, \u03b2\u2010catenin, MITF, and tyrosinase was increased in the RBE\/EMF group, as demonstrated by RT\u2010qPCR analysis. HMB45 and Fontana\u2013Masson immunostaining showed that the RBE\/EMF group had the highest melanin content. Therefore, RBE and EMF may be used as a material and therapy, respectively, for the treatment of vitiligo and white hair, through activation of melanogenesis in melanocytes. Bioelectromagnetics. 39:595\u2013603, 2018. \u00a9 2018 The Authors. <jats:italic>Bioelectromagnetics<\/jats:italic> Published by Wiley Periodicals, Inc..<\/jats:p><\/jats:sec>","DOI":"10.1002\/bem.22151","type":"journal-article","created":{"date-parts":[[2018,10,29]],"date-time":"2018-10-29T14:12:12Z","timestamp":1540822332000},"page":"595-603","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Synergistic effect of rice bran extract and extremely low\u2010frequency electromagnetic fields on dermal papilla\/melanocytes in melanogenesis"],"prefix":"10.1002","volume":"39","author":[{"given":"Soon\u2010Joung","family":"Kwon","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology (BK21 Plus team) Dongguk University Gyeonggi\u2010Do Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology (BK21 Plus team) Dongguk University Gyeonggi\u2010Do Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun\u2010Jun","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology (BK21 Plus team) Dongguk University Gyeonggi\u2010Do Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7533-9605","authenticated-orcid":false,"given":"Young\u2010Kwon","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology (BK21 Plus team) Dongguk University Gyeonggi\u2010Do Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.13.s8.28.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19056202"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033021"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1994.tb08586.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13770-016-0090-2"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2000.130407.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.01.016"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02623492"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"2739","DOI":"10.1242\/jcs.107.10.2739","article-title":"Role of integrins in melanocyte attachment and dendricity","volume":"107","author":"Hara M","year":"1994","journal-title":"J Cell Sci"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(01)00145-1"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3137486"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.5070\/D36GZ420MW","article-title":"The control of hair growth","volume":"4","author":"Jankovic SM","year":"1998","journal-title":"Dermatol Online J"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmr078"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01379.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18102120"},{"key":"e_1_2_7_17_1","first-page":"25","article-title":"Vitamin B12 activates the Wnt\u2010pathway in human hair follicle cells by induction of beta\u2010catenin and inhibition of glycogensynthase kinase\u20103 transcription. Journal of Cosmetics","volume":"1","author":"Krugluger W","year":"2011","journal-title":"Dermatol Sci Appl"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00029330-200602020-00002"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-009-0355-3"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12515907"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2007.00417.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-14-166"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08540.x"},{"key":"e_1_2_7_24_1","first-page":"885","article-title":"Suppression of type I collagen in human scleral fibroblasts treated with extremely low\u2010frequency electromagnetic fields","volume":"19","author":"Wang J","year":"2013","journal-title":"Mol Vis"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2013.46.7.250"}],"container-title":["Bioelectromagnetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbem.22151","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bem.22151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T03:22:49Z","timestamp":1694748169000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bem.22151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,29]]},"references-count":24,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["10.1002\/bem.22151"],"URL":"https:\/\/doi.org\/10.1002\/bem.22151","archive":["Portico"],"relation":{},"ISSN":["0197-8462","1521-186X"],"issn-type":[{"value":"0197-8462","type":"print"},{"value":"1521-186X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,29]]},"assertion":[{"value":"2018-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}