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Heterogenous vortex magnetic fields (VMFs), a new approach of exposure to magnetic fields, induce systematic movements on charged biomolecules from target cells; however, the effect of VMFs on living systems remains uncertain. Here, we designed, constructed, and characterized an ELF\u2010VMF\u2010modified Rodin's coil to expose SH\u2010SY5Y cells. Samples were analyzed by performing 2D\u2010differential\u2010gel electrophoresis, identified by MALDI\u2010TOF\/TOF, validated by western blotting, and characterized by confocal microscopy. A total of 106 protein spots were differentially expressed; 40 spots were downregulated and 66 were upregulated in the exposed cell proteome, compared to the control cell proteome. The identified spots are associated with cytoskeleton and cell viability proteins, and according to the protein\u2013protein interaction network, a significant interaction among them was found. Our data revealed a decrease in cell survival associated with apoptotic cells without effects on the cell cycle, as well as evident changes in the cytoskeleton. We demonstrated that ELF\u2010VMFs, at a specific frequency and exposure time, alter the cell proteome and structurally affect the target cells. This is the first report showing that VMF application might be a versatile system for testing different hypotheses in living systems, using appropriate exposure parameters.\u00a9 2022 Bioelectromagnetics Society.<\/jats:p>","DOI":"10.1002\/bem.22400","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T08:48:27Z","timestamp":1650358107000},"page":"225-244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An Extremely Low\u2010Frequency Vortex Magnetic Field Modifies Protein Expression, Rearranges the Cytoskeleton, and Induces Apoptosis of a Human Neuroblastoma Cell Line"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0036-7227","authenticated-orcid":false,"given":"Diana I.","family":"Aparicio\u2010Bautista","sequence":"first","affiliation":[{"name":"Laboratorio de Estructura de Prote\u00ednas Instituto Nacional de Medicina Gen\u00f3mica  Ciudad 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