{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T06:13:49Z","timestamp":1769840029153,"version":"3.49.0"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T00:00:00Z","timestamp":1668470400000},"content-version":"vor","delay-in-days":45,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["465259\/2014\u20106"],"award-info":[{"award-number":["465259\/2014\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Bioelectromagnetics"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:p>Periodontitis is an inflammatory disease resulting from a complex polymicrobial infection that causes tissue destruction in susceptible individuals. Osteoporosis has been associated with greater clinical attachment loss in patients with periodontitis. Experimental studies have shown positive results in the treatment of osteoporosis through pulsed electromagnetic field (PEMF) stimulation. The aim of this study was to evaluate the effects of PEMF in the presence of estrogen deficiency associated with periodontitis, verifying its role in bone metabolism and in the inflammatory response. Sixty rats were divided into four groups: Sham surgery\u2009+\u2009ligature\u2010induced periodontitis (P); Sham surgery\u2009+\u2009ligature\u2010induced periodontitis\u2009+\u2009PEMF therapy (P\u2009+\u2009PEMF); Ovariectomy surgery\u2009+\u2009ligature\u2010induced periodontitis (P\u2009+\u2009OVX); Ovariectomy surgery\u2009+\u2009ligature\u2010induced periodontitis\u2009+\u2009PEMF therapy (P\u2009+\u2009OVX\u2009+\u2009PEMF). The area of bone loss in the furcation region (BL), connective tissue attachment loss (CTAL) and alveolar bone loss (ABL), BV\/TV and BMD were evaluated. In addition to immunohistochemical labelling of RANKL, OPG, and TRAP and the inflammatory response of interleukin (IL)\u20101b, IL\u20106, TNF\u2010\u03b1, IL\u201010, and vascular endothelial growth factor. P\u2009+\u2009OVX showed significant BL in relation to P\u2009+\u2009PEMF and the greatest CTAL and ABL. P\u2009+\u2009OVX and P\u2009+\u2009OVX\u2009+\u2009PEMF showed a significant reduction in BV\/TV (%). P and P\u2009+\u2009PEMF showed a significantly lesser amount of Tb.Sp (mm) while P\u2009+\u2009OVX and P\u2009+\u2009OVX\u2009+\u2009PEMF showed a lesser of Tb.N. P\u2009+\u2009PEMF had the greatest BMD. P\u2009+\u2009OVX presented higher RANKL and lower OPG immunolabeling than other groups. P\u2009+\u2009PEMF and P\u2009+\u2009OVX\u2009+\u2009PEMF showed a reduction on all biomarkers evaluated. The application of PEMF seems to attenuate the effects of bone loss in the presence of periodontitis and ovariectomy. \u00a9 2022 Bioelectromagnetics Society.<\/jats:p>","DOI":"10.1002\/bem.22424","type":"journal-article","created":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T07:21:27Z","timestamp":1667200887000},"page":"426-437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Effects of the Pulsed Electromagnetic Fields on Experimental Periodontitis and Estrogen Deficiency"],"prefix":"10.1002","volume":"43","author":[{"given":"Daniella V.","family":"Bernardo","sequence":"first","affiliation":[{"name":"Department Diagnosis and Surgery, Division of Periodontics, Institute of Science and Technology UNESP\u2014S\u00e3o Paulo State University S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila L.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department Diagnosis and Surgery, Division of Periodontics, Institute of Science and Technology UNESP\u2014S\u00e3o Paulo State University S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilla M. 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