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In this study, we evaluate the effects of pulsed electromagnetic field (PEMF), a noninvasive low\u2010frequency signal with potential anti\u2010inflammatory effects, in male Swiss mice after a complete Freund's adjuvant (CFA)\u2010induced inflammation. The aim was to identify the optimal PEMF pulse frequency (5, 50, or 75\u2009Hz) and treatment duration (10, 20, or 30\u2009min) for analgesic effects, assessed by mechanical hyperalgesia, and to investigate peripheral (paw) and central (spinal cord, hippocampus, and prefrontal cortex) inflammatory and oxidative responses following CFA administration. Emotion\u2010related behaviors were also assessed; however, CFA did not induce detectable anxiety\u2010 or depression\u2010like alterations under the experimental conditions used. The best antihyperalgesic effect was at 75\u2009Hz, particularly with 20 and 30\u2009min of treatment, without affecting paw edema. A 20\u2010min PEMF treatment at 75\u2009Hz reduced TNF levels in the paw and increased SOD enzyme activity in the paw and spinal cord, indicating anti\u2010inflammatory and antioxidant effects both peripherally and centrally. These results confirm PEMF's antihyperalgesic effect, identifying 75\u2009Hz and 20\u2009min as optimal parameters. Understanding PEMF mechanisms could lead to new therapeutic targets, improving pain management in patients with inflammatory conditions, and altering the underlying pathological process.<\/jats:p>","DOI":"10.1002\/bem.70044","type":"journal-article","created":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T11:21:33Z","timestamp":1769253693000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of Pulsed Electromagnetic Field Parameters Attenuates Mechanical Hyperalgesia and Selectively Modulates Inflammatory and Oxidative Markers in a CFA\u2010Induced Inflammatory Pain Mouse Model"],"prefix":"10.1002","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2412-1602","authenticated-orcid":false,"given":"Jennifer","family":"Hummel","sequence":"first","affiliation":[{"name":"Experimental Neuroscience Laboratory (LaNEx), Postgraduate Program in Health Sciences University of South Santa Catarina 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