{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:15:47Z","timestamp":1792289747735,"version":"4.4.0"},"reference-count":68,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T00:00:00Z","timestamp":1605571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioEssays"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Severe acute respiratory syndrome\u2010coronavirus (SARS\u2010CoV)\u20102\u2032s origin is still controversial. Genomic analyses show SARS\u2010CoV\u20102 likely to be chimeric, most of its sequence closest to bat CoV RaTG13, whereas its receptor binding domain (RBD) is almost identical to that of a pangolin CoV. Chimeric viruses can arise <jats:italic>via<\/jats:italic> natural recombination or human intervention. The furin cleavage site in the spike protein of SARS\u2010CoV\u20102 confers to the virus the ability to cross species and tissue barriers, but was previously unseen in other SARS\u2010like CoVs. Might genetic manipulations have been performed in order to evaluate pangolins as possible intermediate hosts for bat\u2010derived CoVs that were originally unable to bind to human receptors? Both cleavage site and specific RBD could result from site\u2010directed mutagenesis, a procedure that does not leave a trace. Considering the devastating impact of SARS\u2010CoV\u20102 and importance of preventing future pandemics, researchers have a responsibility to carry out a thorough analysis of all possible SARS\u2010CoV\u20102 origins.<\/jats:p>","DOI":"10.1002\/bies.202000240","type":"journal-article","created":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T10:45:39Z","timestamp":1605609939000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["The genetic structure of SARS\u2010CoV\u20102 does not rule out a laboratory origin"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2566-7042","authenticated-orcid":false,"given":"Rossana","family":"Segreto","sequence":"first","affiliation":[{"name":"Department of Microbiology University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Deigin","sequence":"additional","affiliation":[{"name":"Youthereum Genetics Inc.  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,17]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202000091"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0820-9"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00411-20"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-016-3713-9"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2006.131022"},{"key":"e_1_2_13_8_1","unstructured":"Xu L.(2013).The analysis of 6 patients with severe pneumonia caused by unknown viruses (Master's Thesis). Kunming Medical University Emergency Medicine (professional degree).http:\/\/eng.oversea.cnki.net\/Kcms\/detail\/detail.aspx?filename=1013327523.nh&dbcode=CMFD&dbname=CMFD2014"},{"key":"e_1_2_13_9_1","unstructured":"Huang C.(2016).Novel virus discovery in bat and the exploration of receptor of bat coronavirus HKU9 (PhD Thesis). Chinese Center for Disease Control and Prevention.http:\/\/eng.oversea.cnki.net\/kcms\/detail\/detail.aspx?dbcode=CDFD&dbname=CDFDLAST2018&filename=1017118517.nh"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11040379"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1725399"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.369.6503.487"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11110979"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4389089"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-019-1182-0"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006698"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11030210"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12711"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01394-09"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3985"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2017.12.009"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1601512113"},{"key":"e_1_2_13_23_1","first-page":"269","article-title":"Lessons to be learned from recent biosafety incidents in the United States","volume":"17","author":"Weiss S.","year":"2015","journal-title":"Isr. Med. Assoc. J."},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00064-14"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00221-18"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0305-x"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2019.1581171"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00948-15"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.3.1393-1406.2000"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2438-7"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808116105"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1517719113"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25731"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2169-0"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2313-x"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.03.022"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2020.104742"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0688-y"},{"key":"e_1_2_13_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-020-00212-7"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1756700"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2020.04.022"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20133193"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03079-15"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkf469"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00790-20"},{"key":"e_1_2_13_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.05.023"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9716"},{"key":"e_1_2_13_48_1","article-title":"Questions concerning the proximal origin of SARS\u2010CoV\u20102","author":"Seyran M.","year":"2020","journal-title":"J. Med. Virol."},{"key":"e_1_2_13_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2018.12.007"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00127-20"},{"key":"e_1_2_13_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0118-9"},{"key":"e_1_2_13_52_1","first-page":"10","article-title":"Identification of a SARS\u2010like bat coronavirus that shares structural features with the spike glycoprotein receptor\u2010binding domain of SARS\u2010CoV\u20102","author":"Fraguas Bringas C.","year":"2020","journal-title":"Access Microbiol."},{"key":"e_1_2_13_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-020-00660-x"},{"key":"e_1_2_13_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2014.05.026"},{"key":"e_1_2_13_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/v11100972"},{"key":"e_1_2_13_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2507"},{"key":"e_1_2_13_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12626"},{"key":"e_1_2_13_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0225-9"},{"key":"e_1_2_13_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0155-5"},{"key":"e_1_2_13_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10040185"},{"key":"e_1_2_13_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2179-y"},{"key":"e_1_2_13_62_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011184"},{"key":"e_1_2_13_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-019-03863-z"},{"key":"e_1_2_13_64_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032565"},{"key":"e_1_2_13_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature.2017.21487"},{"key":"e_1_2_13_66_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2612.201664"},{"key":"e_1_2_13_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30418-9"},{"key":"e_1_2_13_68_1","author":"Cyranoski D.","year":"2020","journal-title":"Nature"},{"key":"e_1_2_13_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2294-9"}],"container-title":["BioEssays"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bies.202000240","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bies.202000240","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bies.202000240","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T19:27:34Z","timestamp":1693423654000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bies.202000240"}},"subtitle":["SARS\u2010COV\u20102 chimeric structure and furin cleavage site might be the result of genetic manipulation"],"short-title":[],"issued":{"date-parts":[[2020,11,17]]},"references-count":68,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1002\/bies.202000240"],"URL":"https:\/\/doi.org\/10.1002\/bies.202000240","archive":["Portico"],"relation":{},"ISSN":["0265-9247","1521-1878"],"issn-type":[{"value":"0265-9247","type":"print"},{"value":"1521-1878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,17]]},"assertion":[{"value":"2020-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2000240"}}