{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:35:33Z","timestamp":1791725733734,"version":"4.3.4"},"reference-count":81,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioEssays"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CRISPR\u2010Cas technology accelerates development of fast, accurate, and portable diagnostic tools, typified by recent applications in COVID\u201019 diagnosis. Parasitic helminths cause devastating diseases afflicting 1.5 billion people globally, representing a significant public health and economic burden, especially in developing countries. Currently available diagnostic tests for worm infection are neither sufficiently sensitive nor field\u2010friendly for use in low\u2010endemic or resource\u2010poor settings, leading to underestimation of true prevalence rates. Mass drug administration programs are unsustainable long\u2010term, and diagnostic tools \u2013 required to be rapid, specific, sensitive, cost\u2010effective, and user\u2010friendly without specialized equipment and expertise \u2013 are urgently needed for rapid mapping of helminthic diseases and monitoring control programs. We describe the key features of the CRISPR\u2010Cas12\/13 system and emphasise its potential for the development of effective tools for the diagnosis of parasitic and other neglected tropical diseases (NTDs), a key recommendation of the NTDs 2021\u20132030 roadmap released by the World Health Organization.<\/jats:p>","DOI":"10.1002\/bies.202100286","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T12:06:11Z","timestamp":1644494771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Potential of the CRISPR\u2010Cas system for improved parasite diagnosis"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8790-7793","authenticated-orcid":false,"given":"Hong","family":"You","sequence":"first","affiliation":[{"name":"Immunology Department QIMR Berghofer Medical Research Institute  Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Immunology Department QIMR Berghofer Medical Research Institute  Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Skye R.","family":"MacGregor","sequence":"additional","affiliation":[{"name":"Immunology Department QIMR Berghofer Medical Research Institute  Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Cai","sequence":"additional","affiliation":[{"name":"Immunology Department QIMR Berghofer Medical Research Institute  Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald P.","family":"McManus","sequence":"additional","affiliation":[{"name":"Immunology Department QIMR Berghofer Medical Research Institute  Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005355"},{"key":"e_1_2_10_3_1","unstructured":"World Health Organization Team Control of Neglected Tropical Diseases. (2021).Ending the neglect to attain the Sustainable Development Goals: A road map for neglected tropical diseases 2021\u20132030. (Eds:Malecel MweleceleNtuli)World Health Organization.https:\/\/www.who.int\/publications\/i\/item\/9789240010352."},{"key":"e_1_2_10_4_1","unstructured":"World Health Orgnization. (2021).Public consultation: Target product profiles for diagnostic tests to meet schistosomiasis and soil\u2010transmitted helminth programme needs."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq0179"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam9321"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aas8836"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30254-1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra012396"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed4010040"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-018-0390-y"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182017000907"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004318"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.01.006"},{"key":"e_1_2_10_16_1","unstructured":"World Health Organization.(2020).COVID\u201019: WHO issues interim guidance for implementation of NTD programmes."},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18105403"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.0c01984"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-019-09489-1"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2532"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.113.155879"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2641"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00427-014-0486-8"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00427-020-00651-y"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37036-1"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006675"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.113.156299"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008627"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.41463"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.41337"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202001745RR"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.16031.1"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2020.12.011"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2010196117"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2021.117553"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-021-04709-2"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.btre.2020.e00485"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-021-00247-w"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202000185"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2018.12.005"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2021.06.005"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5LC01388G"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2014.908710"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-019-0210-2"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19097-x"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1089\/crispr.2019.0011"},{"key":"e_1_2_10_47_1","unstructured":"Zhang F. Abudayyeh O. A. &Gootenberg J. S.(2020).A protocol for detection of COVID\u201019 using CRISPR diagnostics.https:\/\/www.broadinstitute.org\/files\/publications\/special\/COVID\u201019%20detection%20(updated).pdf."},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.26889"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.12.001"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02001-8"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2279-8"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.1c00107"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-020-0546-5"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1039\/C0CC03957H"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10071655"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.10.024"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2019.08.011"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics10020114"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0513-4"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2020.198129"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13030420"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0908-x"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c00013"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw5122"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18575-6"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2026172"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2010254117"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines9030239"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.627679"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abh2944"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.130411"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094842"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13030466"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103415"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222212577"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1674-8301(11)60001-8"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2020.107458"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)30324-6"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz418"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00673-5"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00760-7"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq0180"}],"container-title":["BioEssays"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bies.202100286","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bies.202100286","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bies.202100286","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T10:44:01Z","timestamp":1692873841000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bies.202100286"}},"subtitle":["CRISPR\u2010Cas mediated diagnosis in parasitic infections"],"short-title":[],"issued":{"date-parts":[[2022,2,10]]},"references-count":81,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1002\/bies.202100286"],"URL":"https:\/\/doi.org\/10.1002\/bies.202100286","archive":["Portico"],"relation":{},"ISSN":["0265-9247","1521-1878"],"issn-type":[{"value":"0265-9247","type":"print"},{"value":"1521-1878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,10]]},"assertion":[{"value":"2021-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2100286"}}