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Under aerobic conditions yeast cells use mainly fermentation of glucose to produce ethanol. Only when glucose is exhausted, cells switch to full respiration of the produced ethanol. We hypothesize that only a subset of the cells continue dividing and switch to respiration. A change from exponential to linear growth is consistent with asymmetrical cell division, where a senescing mother cell produces quiescent daughter cells. We thus propose that most cells produced during fermentation are \u201csomatic,\u201d that is, they rapidly lose reproductive capacity, while the cells continuing to divide constitute the germline, as they exclusively produce rejuvenated quiescent cells. We discuss biased DNA\u2010template strand inheritance by the mother cell as a potential adaptive explanation for germline sequestration to reduce the mutation rate.<\/jats:p>","DOI":"10.1002\/bies.70003","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T12:38:55Z","timestamp":1746189535000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Can Unicellular Organisms Sequester a Germline? The Yeast\u2010Germline Hypothesis"],"prefix":"10.1002","volume":"47","author":[{"given":"Bhavya Sree","family":"Vadlamudi","sequence":"first","affiliation":[{"name":"Plant Sciences Group, Laboratory of Genetics Wageningen University  Wageningen the Netherlands"},{"name":"Agrotechnology &amp; Food Sciences Group, Laboratory of Systems and Synthetic Biology Wageningen University  Wageningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5702-1617","authenticated-orcid":false,"given":"Duur K.","family":"Aanen","sequence":"additional","affiliation":[{"name":"Plant Sciences Group, Laboratory of Genetics Wageningen University  Wageningen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.5962\/bhl.title.88042"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201600125"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/sageke.2001.1.cp13"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.0020141"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300010284"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2005439"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010024\u201003337\u20100"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030045"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097\u20100061(199910)15:14<1511::aid\u2010yea482>3.3.co;2\u2010p"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2005.02.001"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1210663109"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.07.033"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.114.163188"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/1831751a0"},{"volume-title":"Metabolism and Molecular Physiology of Saccharomyces cerevisiae","year":"2002","author":"Dickinson J.","key":"e_1_2_7_16_1"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/ec.3.2.331\u2010338.2004"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3791\/58192"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2019.0128"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07282"},{"key":"e_1_2_7_21_1","first-page":"89","article-title":"Non\u2010Random Assortment of Sister Chromatids in Yeast Mitosis","author":"Williamson D. 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