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These repair mechanisms are conserved across eukaryotic evolution. Repair depends on 5\u2019 to 3\u2019 resection of the broken ends, allowing the assembly of a Rad51 filament. In yeast, fruit flies, and mammals, broken chromosome ends display increased mobility, allowing the ends to explore a larger volume. The Arp2\/3 actin nucleator complex plays a key role in increased local chromatin mobility near a DSB. In budding yeast, Arp2\/3, LAS17\n                    <jats:sup>WASP<\/jats:sup>\n                    , and myosins are required both to initiate and to maintain long\u2010range end resection, affecting both DNA repair and the maintenance of the DNA damage checkpoint. At least in budding yeast, the connection between controlling long\u2010range resection and increased mobility is direct, as inhibiting resection prevents damage\u2010induced chromatin mobility. Moreover, although the search for homology by Rad51\u2010coated broken DNA ends would seem to be improved by greater exploration of the nuclear volume, increased local mobility through Arp2\/3 per se does not affect the efficiency of ectopic recombination in yeast. Here, we review the recently defined roles of nuclear actin in DNA damage repair, with a focus on new observations in budding yeast.\n                  <\/jats:p>","DOI":"10.1002\/bies.70171","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T06:59:33Z","timestamp":1789023573000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Role of Arp2\/3 in End\u2010Resection During DNA Double\u2010Strand Break Repair"],"prefix":"10.1002","volume":"48","author":[{"given":"Felix Y.","family":"Zhou","sequence":"first","affiliation":[{"name":"Rosenstiel Center and Department of Biology Brandeis University  Waltham Massachusetts USA"},{"name":"Radiation Oncology Department Dana Farber Cancer Institute  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Haber","sequence":"additional","affiliation":[{"name":"Rosenstiel Center and 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