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In vertebrates, centromere identity is epigenetically defined by the histone H3 variant CENP\u2010A, whose nucleosomes are replenished during early G1 phase through a cell cycle\u2010regulated deposition mechanism. The CENP\u2010A chaperone HJURP is recruited to centromeres via the Mis18 complex to enable CENP\u2010A incorporation (Mis18C pathway). Recent genetic analyses in chicken cells, however, revealed an additional, independent recruitment route via direct HJURP\u2013CENP\u2010C interaction (CENP\u2010C pathway). Structural studies show that CENP\u2010C and the Mis18C subunit KNL2\/M18BP1 engage pre\u2010existing CENP\u2010A nucleosomes at different surfaces of the Constitutive Centromere\u2010Associated Network (CCAN), constraining the spatial coordinates where new CENP\u2010A is incorporated. This review summarizes current understanding of HJURP recruitment mechanisms for vertebrate CENP\u2010A deposition and proposes that this structure\u2010defined deposition geometry underlies epigenetic self\u2010propagation of centromere positional information by maintaining proper CCAN spacing across cell divisions.<\/jats:p>","DOI":"10.1002\/bies.70177","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T11:08:33Z","timestamp":1787569713000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual Roads of CENP\u2010A for Centromere Identification"],"prefix":"10.1002","volume":"48","author":[{"given":"Tetsuya","family":"Hori","sequence":"first","affiliation":[{"name":"Graduate School of Frontier Biosciences The University of Osaka  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuo","family":"Fukagawa","sequence":"additional","affiliation":[{"name":"Graduate School of Frontier Biosciences The University of Osaka  Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2007.11.009"},{"key":"e_1_2_12_3_1","first-page":"1153","article-title":"A Functional Marker Centromere With no Detectable Alpha\u2010Satellite, Satellite III, or CENP\u2010B Protein: Activation of a Latent Centromere?","volume":"52","author":"Voullaire L. 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