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Early biochemical studies of mammalian DNA repair <jats:italic>in vitro<\/jats:italic> are now at hand. Repair synthesis in damaged DNA can be monitored by following the incorporation of radiolabelled nucleotides. Synthesis is carried out by mammalian cell extracts and is defective in extracts from cell lines derived from individuals with the excisionrepair disorder xeroderma pigmentosum. Biochemical complementation of the defective extracts can be used to purify repair proteins. Repair of damage caused by agents including ultraviolet irradiation, psoralens, and platinating compounds has been observed. Neutralising antibodies against the human single\u2010stranded DNA binding protein (HSSB) have demonstrated a requirement for this protein in DNA excision repair as well as in DNA replication.<\/jats:p>","DOI":"10.1002\/bies.950130904","type":"journal-article","created":{"date-parts":[[2005,2,25]],"date-time":"2005-02-25T10:26:51Z","timestamp":1109327211000},"page":"447-453","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["DNA excision repair in mammalian cell extracts"],"prefix":"10.1002","volume":"13","author":[{"given":"Richard D.","family":"Wood","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn","family":"Coverley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,5]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"DNA repair","author":"Friedberg E.","year":"1985"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1110(90)90022-4"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.54.1.18-51.1990"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"20697","DOI":"10.1016\/S0021-9258(19)47119-6","article-title":"UvrABC Incision of N\u2010methylmitomycin A\u2010DNA monoadducts and cross\u2010links","volume":"264","author":"Pu W. 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