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In this study, the fluorescent (FL) probe of CdTe quantum dots (QDs) was designed using covalently linked rabbit anti\u2010AIV H5N1 antibody. Based on these QD\u2013antibody conjugates, a novel sandwich FL\u2010linked immunosorbent assay (sFLISA) was developed for H5N1 viral antigen detection. The sFLISA allowed for H5N1 viral antigen determination in a linear range of 8.0 \u00d7 10<jats:sup>\u22123<\/jats:sup> to 5.1 \u00d7 10<jats:sup>\u22121<\/jats:sup>\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup> with the limit of detection (LOD) of 1.5 \u00d7 10<jats:sup>\u22124<\/jats:sup>\u2009\u03bcg\u2009mL<jats:sup>\u22121<\/jats:sup>. In comparison with virus isolation for 103 clinic samples, the sensitivity and specificity of sFLISA were found to be 93.6 and 91.1% respectively. The sFLISA supplied a novel approach to rapid and sensitive detection of AIV subtype H5N1 and showed great potential for biological applications in immunoassays. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bio.1167","type":"journal-article","created":{"date-parts":[[2009,10,21]],"date-time":"2009-10-21T03:37:48Z","timestamp":1256096268000},"page":"419-423","source":"Crossref","is-referenced-by-count":68,"title":["Quantum\u2010dots\u2010based fluoroimmunoassay for the rapid and sensitive detection of avian influenza virus subtype H5N1"],"prefix":"10.1002","volume":"25","author":[{"given":"Liping","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonghai","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anding","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuebo","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiakui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heyou","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meilin","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,30]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1204","volume":"46","author":"Isolation of avian influenza A(H5N1) viruses from humans \u2013 Hong Kong, May\u2013December 1997","year":"1997","journal-title":"MMWR Morb. 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