{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T22:29:37Z","timestamp":1759530577929},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,30]],"date-time":"2005-03-30T00:00:00Z","timestamp":1112140800000},"content-version":"vor","delay-in-days":5842,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biolumin. Chemilumin."],"published-print":{"date-parts":[[1989,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated whether polymorphonuclear leukocytes (PMN) are able to kill human neuroblastoma cells either directly or if coated with antibody MAb 14.18 that recognizes ganglioside GD<jats:sub>2<\/jats:sub> present on the cell surface of most neuroblastoma cells. Neuroblastoma cells could not be destroyed directly, whereas in the antibody\u2010dependent reaction (ADCC\u2010reaction) they were easily eliminated. In order to answer the question whether reactive oxygen intermediates are involved in this process, chemiluminescence measurements were performed. Compared to the signals that could be measured using opsonized zymosan as stimulus, only weak CL\u2010signals could be registered during the ADCC reaction. Pretreatment of PMN with granulocyte\u2010macrophage colony stimulating factor (GM\u2010CSF) enhanced the CL\u2010signals, catalase and SOD reduced it; however, cell killing was only slightly influenced in the presence of catalase and superoxide dismutase. These data suggested that reactive oxygen compounds do not play a prominent role in the killing process. Definitive evidence for this suggestion could be obtained using PMN from a patient with chronic granulomatous disease (CGD): MAb 14.18 coated neuroblastoma cells could be killed effectively, but no CL\u2010signal could be registered, either in the ADCC\u2010reaction or using opsonized zymosan as stimulus.<\/jats:p>","DOI":"10.1002\/bio.1170030212","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T00:03:42Z","timestamp":1117325022000},"page":"93-96","source":"Crossref","is-referenced-by-count":10,"title":["Effects of granulocytes on human neuroblastoma cells measured by chemiluminescence and chromium\u201051 release assay"],"prefix":"10.1002","volume":"3","author":[{"given":"Gernot","family":"Bruchelt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupert","family":"Handgretinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Astrid","family":"Kimmig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Goeke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruben","family":"Siedner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph A.","family":"Reisfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dietrich","family":"Niethammer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rn","family":"Treuner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.4049\/jimmunol.122.6.2605","article-title":"Role of the myelo\u2010peroxidase\u2010H2O2\u2010halidade system in concanavalin A\u2010induced tumor cell killing by human neutrophils","volume":"122","author":"Clark R. A.","year":"1979","journal-title":"J. Immunol."},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.4049\/jimmunol.122.6.2605","article-title":"Role of the myelo\u2010peroxidase\u2010H2O2\u2010halidade system in concanavalin A\u2010induced tumor cell killing by human neutrophils","volume":"122","author":"Clark R. A.","year":"1979","journal-title":"J. Immunol."},{"key":"e_1_2_1_4_1","first-page":"273","article-title":"Mechanisms of tumor cell destruction by PMA\u2010activated human neutrophils","volume":"48","author":"Dallegri F.","year":"1983","journal-title":"Immunology"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1182\/blood.V54.2.407.407","article-title":"Cytotoxicity activity of human monocytes towards sensitized red cells is not dependent on the generation of reactive oxygen species","volume":"54","author":"Fleer A. D.","year":"1979","journal-title":"Blood"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.4049\/jimmunol.123.1.55","article-title":"Polymorphonuclear leukocyte\u2010mediated, antibody\u2010dependent, cellular cytotoxicity against tumor cells: dependence on oxygen and the respiratory burst","volume":"123","author":"Hafeman D. G.","year":"1979","journal-title":"J. Immunol."},{"key":"e_1_2_1_7_1","first-page":"116","volume-title":"Haematology and Blood Transfusion","author":"Handgretinger R.","year":"1987"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(81)90040-5"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.4049\/jimmunol.127.4.1585","article-title":"Interferon enhances the antibody\u2010dependent cellular cytotoxicity (ADCC) of human polymorphonuclear leukocytes","volume":"127","author":"Hokland P.","year":"1981","journal-title":"J. Immunol."},{"key":"e_1_2_1_10_1","first-page":"2211","article-title":"Stimulation of the respiratory burst of murine peritoneal inflammatory neutrophils by conjugation with tumor cells","volume":"47","author":"Lichtenstein A.","year":"1987","journal-title":"Cancer Res."},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"242","DOI":"10.4049\/jimmunol.135.1.242","article-title":"Human neuroblastoma cell lines are susceptible to lysis by natural killer cells but not by cytotoxic T lymphocytes","volume":"135","author":"Main E. J.","year":"1985","journal-title":"J. Immunol."}],"container-title":["Journal of Bioluminescence and Chemiluminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbio.1170030212","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.1170030212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T03:25:57Z","timestamp":1697945157000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bio.1170030212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,4]]}},"alternative-id":["10.1002\/bio.1170030212"],"URL":"https:\/\/doi.org\/10.1002\/bio.1170030212","archive":["Portico"],"relation":{},"ISSN":["0884-3996","1099-1271"],"issn-type":[{"value":"0884-3996","type":"print"},{"value":"1099-1271","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4]]}}}