{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T16:11:18Z","timestamp":1770999078957,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,9]],"date-time":"2005-04-09T00:00:00Z","timestamp":1113004800000},"content-version":"vor","delay-in-days":5761,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biolumin. Chemilumin."],"published-print":{"date-parts":[[1989,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new series of stable acridinium ester conjugates have been developed for use as non\u2010isotopic labels in immunoassay. They have proved to be a flexible alternative to radioimmunoassay. We present data showing the successful development of immunoassays in sandwich, competitive and receptor formats. In addition, hydrophilic acridinium ester analogues have been synthesized, encapsulated in liposomes, and utilized as labels in immunoassay. The potential of this technology is discussed.<\/jats:p>","DOI":"10.1002\/bio.1170040115","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T00:04:18Z","timestamp":1117325058000},"page":"88-98","source":"Crossref","is-referenced-by-count":41,"title":["Novel poly\u2010substituted aryl acridinium esters and their use in immunoassay"],"prefix":"10.1002","volume":"4","author":[{"given":"Say\u2010Jong","family":"Law","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uri","family":"Piran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol","family":"Klukas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Unger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"7695","DOI":"10.1016\/S0021-9258(19)44580-8","article-title":"Isolation of vitamin B12\u2010binding proteins using affinity chromatography","volume":"247","author":"Allen R. H.","year":"1972","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(65)80093-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsscb.1981.380160305"},{"key":"e_1_2_1_5_1","unstructured":"Davis P. J. (1979). Processes and materials for detecting and determining proteinaceous specific binding agents and materials bindable thereto. Patent: PCT International WO 8001515."},{"key":"e_1_2_1_6_1","first-page":"156","article-title":"BioMag \u2013 a new support for magnetic affinity chromatography","volume":"3","author":"Groman E. V.","year":"1985","journal-title":"Biotechniques"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90221-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1093\/clinchem\/30.2.290","article-title":"How to improve estimates of imprecision","volume":"30","author":"Krouwer J. S.","year":"1984","journal-title":"Clin. Chem."},{"key":"e_1_2_1_9_1","unstructured":"Law S. J.andPiran U. (1988). Method for detection of an analyte using acridinium esters and liposomes. US Patent Application Serial #226 639."},{"key":"e_1_2_1_10_1","unstructured":"Law S. J. Chang S. C. S. Palmacci S. A.andCubicciotti R. S. (1988a). Polysubstituted aryl acridinium esters. US Patent #4 745 181."},{"key":"e_1_2_1_11_1","unstructured":"Law S. J. Chang S. C. S. Klukas C. K. andVitkauskas C. A. (1988b).Nucleophilic polysubstituted aryl acridinium esters and uses thereof. Patent Pending."},{"key":"e_1_2_1_12_1","first-page":"9","volume-title":"Bioluminescence and Chemiluminescence: Instruments and Applications","author":"McCapra F.","year":"1985"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(79)90085-3"},{"key":"e_1_2_1_14_1","first-page":"181","volume-title":"Luminescent Assays","author":"Patel A.","year":"1982"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1093\/clinchem\/33.9.1517","article-title":"Immunochemiluminometric assay of creatine kinase MB with a monoclonal antibody to the MB isoenzyme","volume":"33","author":"Piran U.","year":"1987","journal-title":"Clin. Chem."},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1093\/clinchem\/27.7.1157","article-title":"Alternatives to radioimmunoassay: labels and methods","volume":"27","author":"Schall R. F.","year":"1981","journal-title":"Clin. Chem."},{"key":"e_1_2_1_17_1","first-page":"673","volume-title":"Bioluminescence and Chemiluminescence \u2010 Basic Chemistry and Analytical Applications.","author":"Simpson J. S. A.","year":"1981"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1093\/clinchem\/29.8.1474","article-title":"Acridinium esters as high\u2010specific activity labels in immunoassay","volume":"29","author":"Weeks I.","year":"1983","journal-title":"Clin. Chem."},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.1984.tb03446.x"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-9936(88)85010-6"}],"container-title":["Journal of Bioluminescence and Chemiluminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbio.1170040115","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.1170040115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T04:57:06Z","timestamp":1697950626000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bio.1170040115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,7]]}},"alternative-id":["10.1002\/bio.1170040115"],"URL":"https:\/\/doi.org\/10.1002\/bio.1170040115","archive":["Portico"],"relation":{},"ISSN":["0884-3996","1099-1271"],"issn-type":[{"value":"0884-3996","type":"print"},{"value":"1099-1271","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7]]}}}