{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T13:10:23Z","timestamp":1698066623375},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,4,9]],"date-time":"2005-04-09T00:00:00Z","timestamp":1113004800000},"content-version":"vor","delay-in-days":5761,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biolumin. Chemilumin."],"published-print":{"date-parts":[[1989,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We described a chemiluminescent immunoassay (CIA) for 5\u03b1\u2010androstane\u20103\u03b1, 17\u03b2\u2010diol\u2010glucuronide (3\u03b1\u2010diol\u2010G) in human diluted urine. This method allowed the direct measurement in 1\u03bcl of urine avoiding the hydrolysis and extraction steps for sample pretreatment commonly used in routine methods. The hapten 3\u03b1\u2010diol\u2010G was synthesized by a Koenigs\u2013Knorr reaction. The immunogenic complex, 3\u03b1\u2010diol\u2010G conjugated to bovine serum albumin (BSA), was employed to induce the formation of specific antibodies in New Zealand rabbits. In addition, the required chemiluminescent (CL) tracer was prepared. The characteristics of the antibody was determined as regard to specificity and sensitivity and the precision of the assay methods established. In 22 hirsute women affected by policystic ovarian syndrome we found 3\u03b1\u2010diol\u2010G values significantly (<jats:italic>p<\/jats:italic> &lt; 0.01) higher (146.28 \u00b1 73.77\u03bcg\/g of creatinine; mean \u00b1 SD) than those observed in normal women (72.1 \u00b1 32.58 \u03bcg\/g of creatinine; mean \u00b1 SD).<\/jats:p>","DOI":"10.1002\/bio.1170040175","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T00:04:18Z","timestamp":1117325058000},"page":"575-579","source":"Crossref","is-referenced-by-count":2,"title":["A chemiluminescent immunoassay for the direct measurement of urinary 5\u03b1\u2010androstane\u20103\u03b1, 17\u03b2\u2010diol\u2010glucuronide in urine"],"prefix":"10.1002","volume":"4","author":[{"given":"P.","family":"Pinzani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Franceschetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Magini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Orlandini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Pazzagli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,9]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1090","article-title":"Steroid\u2010protein conjugates: preparation and characterization of conjugates of bovine serum albumin with progesterone, deoxycorticosterone and estrone","volume":"234","author":"Erlanger B. F.","year":"1959","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-21-11-1440"},{"key":"e_1_2_1_4_1","first-page":"47","volume-title":"Androgenization in Women","author":"Mauvais\u2010Jarvis P.","year":"1983"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/S0015-0282(16)49515-4","article-title":"Measurements of 3\u03b1, 17\u03b2\u2010androstanediol gluconide in serum and urine and the correlation with s 5\u03b1\u2010reductase activity","volume":"46","author":"Paulson R. J.","year":"1986","journal-title":"Fert. Steril."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(81)90208-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(86)90255-4"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(80)90353-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","unstructured":"Schroeder H. R.andYeager F. M. (1978).Chemiluminescence yields and detection limits of some isolumi derivatives in various oxidation systems. Anal. Chem. 1114\u20131120.","DOI":"10.1021\/ac50030a027"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)48320-2"},{"key":"e_1_2_1_11_1","first-page":"68","volume-title":"Immunologic Methods in Steroid Determination","author":"Thorneycroft I.","year":"1970"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","unstructured":"Wright F. Mowszowicz I.andMauvais\u2010Jarvis P. (1978).Urinary 5\u03b1\u2010androstane\u20103\u03b1 17\u03b2\u2010diol radioimmunoassay new clinical evaluation. J. Clin. Endocrinol. Metab. 850\u2013854.","DOI":"10.1210\/jcem-47-4-850"}],"container-title":["Journal of Bioluminescence and Chemiluminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbio.1170040175","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.1170040175","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T04:59:18Z","timestamp":1697950758000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bio.1170040175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,7]]}},"alternative-id":["10.1002\/bio.1170040175"],"URL":"https:\/\/doi.org\/10.1002\/bio.1170040175","archive":["Portico"],"relation":{},"ISSN":["0884-3996","1099-1271"],"issn-type":[{"value":"0884-3996","type":"print"},{"value":"1099-1271","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7]]}}}