{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T10:42:10Z","timestamp":1752230530834},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,30]],"date-time":"2005-03-30T00:00:00Z","timestamp":1112140800000},"content-version":"vor","delay-in-days":4471,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Biolumin. Chemilumin."],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of different concentrations (0.06, 0.6 and 6.0 mmol\/L) of ascorbic acid on neutrophil\u2013endothelial interaction was studied using an <jats:italic>in vitro<\/jats:italic> model of human umbilical cord vein endothelial cells and human neutrophils. The aim of the study was to determine changes in chemiluminescence response of neutrophils during adherence to endothelial cells. Because adherence of neutrophils to endothelial cells is an essential component in inflammatory processes leading to endothelial cell injury, the influence of ascorbic acid on adherence and endothelial cell injury have been investigated. Production of oxygen\u2010derived metabolites, measured by chemiluminescence response of neutrophils, decreased significantly in the presence of 6 mmol\/L ascorbic acid during coincubation of neutrophils and endothelial cells (<jats:italic>p<\/jats:italic> &lt; 0.025). The adherence of neutrophils to endothelial cells was significantly decreased at a concentration of 6 mmol\/L (<jats:italic>p<\/jats:italic> &lt; 0.0005). The inhibition of neutrophil adherence to endothelial cells was correlated with a diminished neutrophil\u2010mediated endothelial cell injury during incubation with 6 mmol\/L ascorbic acid (<jats:italic>p<\/jats:italic> &lt; 0.0005). The present results indicate that ascorbic acid might exert a protective effect on neutrophil\u2010mediated endothelial cell injury by decreasing adherence of neutrophils to endothelial cells and by scavenging reactive oxygen metabolites. Moreover, the current investigation points to probable protective effect of ascorbic acid on oxidant\u2010mediated cell damage in diseases (e.g., Adult Respiratory Distress Syndrome).<\/jats:p>","DOI":"10.1002\/bio.1170080104","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T00:09:08Z","timestamp":1117325348000},"page":"15-20","source":"Crossref","is-referenced-by-count":23,"title":["<i>In vitro<\/i> effect of ascorbic acid on neutrophil\u2013endothelial cell 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