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In the presence of glucose oxidase, glucose is oxidized to yield, gluconic acid and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and H<jats:sup>+<\/jats:sup> (dissociated from gluconic acid) intensively quenched the fluorescence of QDs. The experimental results showed that the quenched fluorescence was proportional to the glucose concentration within the range of 0.01\u20135.0\u2009m<jats:sc>m<\/jats:sc> under optimized experimental conditions. Compared with most of the existing methods, this newly developed system possesses many advantages, including simplicity, low cost, high flexibility, and good sensitivity. Furthermore, no complicated chemical modification of QDs and enzyme immobilization was needed in this system. 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