{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T05:08:13Z","timestamp":1789880893763,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T00:00:00Z","timestamp":1354147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Aqueous phase synthesis of CdTe quantum dots (QDs) with surface functionalization for bioconjugation remains the best approach for biosensing and bioimaging applications. We present a facile aqueous phase method to prepare CdTe QDs by adjusting precursor and ligand concentrations. CdTe QDs had photoluminescence quantum yield up to \u224833% with a narrow spectral distribution. The powder X\u2010ray diffraction profile elucidated characteristic broad peaks of zinc blende cubic CdTe nanoparticles with 2.5\u20133\u2009nm average crystalline size having regular spherical morphology as revealed by transmission electron microscopy. Infra\u2010red spectroscopy confirmed disappearance of characteristic absorptions for \u2013SH thiols inferring thiol coordinated CdTe nanoparticles. The effective molar concentration of 1 : 2.5 : 0.5 respectively for Cd<jats:sup>2+<\/jats:sup>\/3\u2010mercaptopropionic acid\/HTe<jats:sup>\u2013<\/jats:sup> at pH 9\u2009\u00b1\u20090.2 resulted in CdTe quantum dots of 2.2\u20133.06\u2009nm having band gap in the range 2.74\u20132.26 eV respectively. Later, QD<jats:sub>523<\/jats:sub> and QD<jats:sub>601<\/jats:sub> were used for monitoring staphylococcal enterotoxin B (SEB; a bacterial superantigen responsible for food poisoning) using Forster resonance energy transfer based two QD fluorescence. QD<jats:sub>523<\/jats:sub> and QD<jats:sub>601<\/jats:sub> were bioconjugated to anti\u2010SEB IgY antibody and SEB respectively according to carbodiimide protocol. The mutual affinity between SEB and anti\u2010SEB antibody was relied upon to obtain efficient energy transfer between respective QDs resulting in fluorescence quenching of QD<jats:sub>523<\/jats:sub> and fluorescence enhancement of QD<jats:sub>601<\/jats:sub>. Presence of SEB in the range 1\u20130.05\u2009\u00b5g varied the rate of fluorescence quenching of QD<jats:sub>523<\/jats:sub>, thereby demonstrating efficient use of QDs in the Forster resonance energy transfer based immunosensing method by engineering the QD size. Copyright \u00a9 2012 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bio.2440","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T07:14:42Z","timestamp":1354173282000},"page":"827-835","source":"Crossref","is-referenced-by-count":21,"title":["Facile synthesis and photophysical characterization of luminescent CdTe quantum dots for Forster resonance energy transfer based immunosensing of staphylococcal enterotoxin B"],"prefix":"10.1002","volume":"28","author":[{"given":"Aaydha C.","family":"Vinayaka","sequence":"first","affiliation":[{"name":"Fermentation Technology &amp; Bioengineering Department Central Food Technological Research Institute  Mysore 570020 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munna S.","family":"Thakur","sequence":"additional","affiliation":[{"name":"Fermentation Technology &amp; Bioengineering Department Central Food Technological Research Institute  Mysore 570020 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