{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T02:11:26Z","timestamp":1774923086344,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,7,5]],"date-time":"2013-07-05T00:00:00Z","timestamp":1372982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We developed a highly sensitive chemiluminescent (CL) assay for hydrogen peroxide using 10\u2010methyl\u20109\u2010(phenoxycarbonyl) acridinium fluorosulfonate (PMAC) that produced chemiluminescence under neutral conditions and applied it to an enzyme immunoassay (EIA). One picomole of hydrogen peroxide could be detected using the optimized PMAC\u2010CL method and 6.2 \u00d7 10<jats:sup>\u201020<\/jats:sup> mol \u03b2\u2010<jats:sc>d<\/jats:sc>\u2010galactosidase (\u03b2\u2010gal) could be detected by combining an indoxyl derivative substrate and the proposed PMAC\u2010CL method. This highly sensitive CL \u03b2\u2010gal assay was applied to an EIA for thyroid\u2010stimulating hormone (TSH) using \u03b2\u2010gal as a label enzyme; 0.02\u2013100.0 \u03bcU\/mL TSH in human serum could be assayed directly and with high reproducibility. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bio.2555","type":"journal-article","created":{"date-parts":[[2013,7,5]],"date-time":"2013-07-05T07:49:08Z","timestamp":1373010548000},"page":"374-377","source":"Crossref","is-referenced-by-count":20,"title":["Development of a highly sensitive chemiluminescent assay for hydrogen peroxide under neutral conditions using acridinium ester and its application to an enzyme immunoassay"],"prefix":"10.1002","volume":"29","author":[{"given":"Hidetoshi","family":"Arakawa","sequence":"first","affiliation":[{"name":"School of Pharmacy Showa University  Shinagawa\u2010ku Tokyo 142\u20108555 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Tsuruoka","sequence":"additional","affiliation":[{"name":"School of Pharmacy Showa University  Shinagawa\u2010ku Tokyo 142\u20108555 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken\u2010ichi","family":"Ohno","sequence":"additional","affiliation":[{"name":"School of Pharmacy Showa University  Shinagawa\u2010ku Tokyo 142\u20108555 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko","family":"Tajima","sequence":"additional","affiliation":[{"name":"School of Pharmacy Showa University  Shinagawa\u2010ku Tokyo 142\u20108555 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Nagano","sequence":"additional","affiliation":[{"name":"School of Pharmacy Showa University  Shinagawa\u2010ku Tokyo 142\u20108555 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.958"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(79)90352-X"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(03)00809-2"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(91)90096-C"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.15.1269"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90017-2"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.1170040121"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar50102a001"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp104060x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.6.645"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.1170080302"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.1170040116"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbio.2555","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.2555","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:10:14Z","timestamp":1759943414000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.2555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,5]]},"references-count":12,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1002\/bio.2555"],"URL":"https:\/\/doi.org\/10.1002\/bio.2555","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,5]]}}}