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The binding parameters were confirmed using fluorescence quenching spectroscopy at various temperatures. The experimental results confirmed that the quenching mechanisms of CL and HSA\/BSA were both static quenching processes. The thermodynamic parameters, namely, enthalpy change (\u0394<jats:italic>H<\/jats:italic>) and entropy change (\u0394<jats:italic>S<\/jats:italic>), were calculated according to the van't Hoff equation, which suggested that the electrostatic interactions were the predominant intermolecular forces in stabilizing the CL\u2013HSA complex, and hydrogen bonds and van der Waals force were the predominant intermolecular forces in stabilizing the CL\u2013BSA complex. Furthermore, the conformational changes of HSA\/BSA in the presence of CL were determined using the data obtained from three\u2010dimensional fluorescence spectroscopy, ultraviolet\u2010visible absorption spectroscopy and circular dichroism spectroscopy. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bio.2574","type":"journal-article","created":{"date-parts":[[2013,9,13]],"date-time":"2013-09-13T05:59:09Z","timestamp":1379051949000},"page":"492-499","source":"Crossref","is-referenced-by-count":6,"title":["Studies on binding interactions between clenbuterol hydrochloride and two serum albumins by multispectroscopic approaches <i>in vitro<\/i>"],"prefix":"10.1002","volume":"29","author":[{"given":"Qin","family":"Wang","sequence":"first","affiliation":[{"name":"School of Chemistry and Environment Science Shaanxi University of Technology  Hanzhong Shaanxi 723000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengrui","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Environment Science Shaanxi University of Technology  Hanzhong Shaanxi 723000 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