{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T13:00:17Z","timestamp":1788872417522,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,5,16]],"date-time":"2014-05-16T00:00:00Z","timestamp":1400198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["20975041,21275056"],"award-info":[{"award-number":["20975041,21275056"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Natural Science Foundation of Guangdong province","award":["10251063101000000"],"award-info":[{"award-number":["10251063101000000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The determination of hormone\u2010binding sites in plants is essential in understanding the mechanisms behind hormone function. Salicylic acid (SA) is an important plant hormone that regulates responses to biotic and abiotic stresses. In order to label SA\u2010binding sites in plant tissues, a quantum dots (QDs) probe functionalized with a SA moiety was successfully synthesized by coupling CdSe QDs capped with 3\u2010mercaptopropionic acid (MPA) to 4\u2010amino\u20102\u2010hydroxybenzoic acid (PAS), using 1\u2010ethyl\u20103\u2010(3\u2010dimethyllaminopropyl) carbodiimide (EDC) as the coupling agent. The probe was then characterized by dynamic light scattering and transmission electron microscopy, as well as UV\/vis and fluorescence spectrophotometry. The results confirmed the successful conjugation of PAS to CdSe QDs and revealed that the conjugates maintained the properties of the original QDs, with small core diameters and adequate dispersal in solution. The PAS\u2013CdSe QDs were used to detect SA\u2010binding sites in mung bean and\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    seedlings in vitro and in vivo. The PAS\u2013CdSe QDs were effectively transported into plant tissues and specifically bound to SA receptors in vivo. In addition, the effects of the PAS\u2013CdSe QDs on cytosolic Ca\n                    <jats:sup>2+<\/jats:sup>\n                    levels in the tips of\n                    <jats:italic>A. thaliana<\/jats:italic>\n                    seedlings were investigated. Both SA and PAS\u2013CdSe QDs had similar effects on the trend in cytosolic\u2010free Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentrations, suggesting that the PAS\u2013CdSe QDs maintained the bioactivity of SA. To summarize, PAS\u2013CdSe QDs have high potential as a fluorescent probe for the in vitro\/in vivo labeling and imaging of SA receptors in plants. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/bio.2682","type":"journal-article","created":{"date-parts":[[2014,5,16]],"date-time":"2014-05-16T04:51:49Z","timestamp":1400215909000},"page":"18-25","source":"Crossref","is-referenced-by-count":13,"title":["In situ detection of salicylic acid binding sites in plant tissues"],"prefix":"10.1002","volume":"30","author":[{"given":"Jing\u2010Wen","family":"Liu","sequence":"first","affiliation":[{"name":"School of Chemistry and Environment South China Normal University  Guangzhou Guangdong 510006 People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da\u2010Yi","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Chemistry and Environment South China Normal University  Guangzhou Guangdong 510006 People's Republic of 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