{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T04:18:58Z","timestamp":1778559538922,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T00:00:00Z","timestamp":1425340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["20875078"],"award-info":[{"award-number":["20875078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Luminescence"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple and sensitive resonance Rayleigh scattering (RRS) spectra method was developed for the determination of calf thymus DNA (ctDNA). The enhanced RRS signals were based on the interactions between ctDNA and aminoglycoside antibiotics (AGs) including kanamycin (KANA), tobramycin (TOB), gentamicin (GEN) and neomycin (NEO) in a weakly acidic medium (pH 3.3\u20135.7). Parameters influencing the method were investigated. Under optimum conditions, increments in the scattering intensity (\u2206<jats:italic>I<\/jats:italic>) were directly proportional to the concentration of ctDNA over certain ranges. The detection limit ranged from 12.2 to 16.9 ng\/mL. Spectroscopic methods, including RRS spectra, absorption spectra and circular dichroism (CD) spectroscopy, coupled with thermo\u2010denaturation experiments were used to study the interactions, indicating that the interaction between AGs with ctDNA was electrostatic binding mode. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bio.2876","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T20:02:27Z","timestamp":1425412947000},"page":"1159-1166","source":"Crossref","is-referenced-by-count":12,"title":["Study on interactions of aminoglycoside antibiotics with calf thymus DNA and determination of calf thymus DNA via the resonance Rayleigh scattering technique"],"prefix":"10.1002","volume":"30","author":[{"given":"Man","family":"Qiao","sequence":"first","affiliation":[{"name":"Key Laboratory of Luminescent and Real\u2010Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Luminescent and Real\u2010Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Luminescent and Real\u2010Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaopu","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Luminescent and Real\u2010Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongfang","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Luminescent and Real\u2010Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Luminescent and Real\u2010Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering Southwest University  Chongqing 400715 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioelechem.2009.07.001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2004.07.039"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac800847r"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2012.08.005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(97)00008-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.02.029"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200900779"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb3005116"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2008.04.015"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2007.05.004"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000095"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmg.2006.04.003"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20052"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cc42140f"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.2443"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.061176"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.08.031"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bio.884"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2011.12.051"},{"key":"e_1_2_6_21_1","first-page":"62","article-title":"Characterization of interaction of DNA with Hoechst 33258 using resonance light scattering spectra and their analytical application","volume":"30","author":"Wang B","year":"2008","journal-title":"J Southwest University (Natural Science Ed)"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11426-007-0046-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2012.12.041"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4236\/ajac.2012.33026"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2010.10.024"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-012-0891-5"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-011-0547-x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2009.12.043"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2004.02.025"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac50064a004"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2005.02.022"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(00)01063-1"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2005.02.053"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2007.01.050"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2004.07.013"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(99)00502-4"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2006.03.030"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(98)00254-2"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-003-0044-y"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm101414w"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2013.06.002"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf1000748"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2010.1054"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-1425(01)00554-6"},{"key":"e_1_2_6_45_1","first-page":"185","article-title":"The determination of polybasic ionization constants of kanamycin A and B","volume":"18","author":"Li Z","year":"2004","journal-title":"J Chem Eng Chinese U"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7638615"},{"key":"e_1_2_6_47_1","first-page":"312","article-title":"Studies on the interaction of chlorpromazine hydrochloride and promethazine hydrochloride with nucleic acid","volume":"35","author":"Liu S","year":"2005","journal-title":"Sci China Chem"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1360\/02yb9024"}],"container-title":["Luminescence"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbio.2876","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bio.2876","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:12:31Z","timestamp":1759932751000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bio.2876"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,3]]},"references-count":47,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1002\/bio.2876"],"URL":"https:\/\/doi.org\/10.1002\/bio.2876","archive":["Portico"],"relation":{},"ISSN":["1522-7235","1522-7243"],"issn-type":[{"value":"1522-7235","type":"print"},{"value":"1522-7243","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,3]]}}}